아데노바이러스 주입 후 백서의 비강과 부비동 점막에서의 발현 양상

유영화; 박용진; 강준명; 강보성; 최진

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자료유형: 학술저널

저자정보: 유영화 박용진 강준명 강보성 최진

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제50권 제10호

발행연도: 2007.1

수록면: 882 - 887 (6page)

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연구주제

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연구배경

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연구방법

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연구결과

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Background and Objectives：A potential use of gene therapy to deliver therapeutic peptides to the nasal mucosa has not ben sal cavity and paranasal sinus mucosa of the rat. Materials and Method：Ten male Sprague Dawley rats were used in this experi-ments, and adenovirus vectors containing gren fluorescent protein (GFP) were injected into rat via tail vein. On fourteen days after gene transfer, 10 rats were sacrificed. The turbinate, nasal septum and paranasal sinus mucosa were examined by fluore-scent microscope, and imunohistochemical analysis using anti-GFP antibody was performed. Results：septal mucosa after injection of adenovirus vector expressing GFP showed difused distribution of fluorescent produced by GFP. Immunohistochemical analysis showed that GFP expresion in the turbinate mucosa was localized to the largely ciliated co-lmnar epithelium and to some lamnina propria, and that in the septal mucosa, it was localized to the ciliated columnar epithelium. Expression in the turbinate mucosa was more obvious than that in the nasal septal mucosa. There was no expression in the paranasal sinus mucosa. Conclusion：turbinate and nasal septal mucosa. Gene therapy targeting mucosal epithelium can be a helpful method to treat patients with chronic inflamatory disease of the nasal mucosa such as alergic rhinitis or neoplasm of the nasal cavity. (Korean J Otor -hinolaryngol-Head Neck Surg 2007 ;50 :882-7)

#녹색형광단백·아데노바이러스·비갑개·비중격·부비동·유전자 치료. #Green fluorescent protein #GFP)· Adenovirus· Turbinate· Nasal septum· Paranasal sinuses· Gene therapy.

참고문헌 (17)

참고문헌 신청

/ / Construction of a defective adenovirus vector expresing the pseudorabies virus glycoprotein gp50 and its use as a live vaccine : 2425 ~ 10 31

/ / Ex-pression paterns of green fluorescent protein after the intra-venous injection with adenovirus vector in mouse organs

/ / Primary structure of the Aequo-rea victoria green-fluorescent protein : 229 ~ 33

/ 1995 / A Controled study of adenoviral-vector-mediated gene transfer in the nasal epithelium of patients with cystic fibrosis 333 (13) : 823 ~ 831

/ 1995 / Expresion of alpha v beta 5 integrin is necessary for eficient adenovirus-mediated gene transfer in the human airway 69 (10) : 5951 ~ 5958

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