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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1 - 6 (6page)
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Background: The objective of this study was to develop an efficient selectable marker for transgenic Dunaliella salina.
Results: Tests of the sensitivity of D. salina to the antibiotic chloramphenicol and the herbicide Basta® showed that cells (1.0 × 106 cells/ml) treated with 1000 or 1500 μg/ml chloramphenicol died in 8 or 6 days, respectively, whereas D. salina cells (1.0 × 106 cells/ml) treated with 5, 10, 20, or 40 μg/ml Basta® died in 2 days. Therefore, D. salina is more sensitive to Basta® than to chloramphenicol. To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells and that the cells survived in 5 μg/ml Basta®, the medium used to select the transformed cells.
Conclusions: The findings of this study suggest that the pat gene can be used as an efficient selectable marker when producing transgenic D. salina.
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참고문헌 (29)
참고문헌 신청
Akbari F / 2014 / The potential of transgenic green microalgae : a robust photobioreactor to produce recombinant therapeutic proteins / World J Microb Biot 30:2783~2796
Banerjee A / 2002 / Botryococcus braunii : a renewable source of hydrocarbons and other chemicals / Crit Rev Biotechnol 22:245~279
Bohorova N. / 1999 / Laboratory protocols: CIMMYT Applied genetic engineering laboratory / International Maize and Wheat Improvement Center :39~43
Borowitzka MA / 2007 / The taxonomy of the genus Dunaliella(Chlorophyta, Dunaliellales)with emphasis on the marine and halophilic species / J Appl Phycol 19:567~590
Cerutti H / 1997 / A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii : integration into the nuclear genome and gene expression / Genetics 145:97~110
Banerjee A / 2002 / Botryococcus braunii : a renewable source of hydrocarbons and other chemicals / Crit Rev Biotechnol 22:245~279
Bohorova N. / 1999 / Laboratory protocols: CIMMYT Applied genetic engineering laboratory / International Maize and Wheat Improvement Center :39~43
Borowitzka MA / 2007 / The taxonomy of the genus Dunaliella(Chlorophyta, Dunaliellales)with emphasis on the marine and halophilic species / J Appl Phycol 19:567~590
Cerutti H / 1997 / A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii : integration into the nuclear genome and gene expression / Genetics 145:97~110
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