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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 27 - 38 (12page)
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The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation.
In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.
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참고문헌 (37)
참고문헌 신청
Bevan MW / 1983 / A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation / Nature 304:184~187
Block DM / 1989 / Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants / Plant Physiol 91:694~701
Breyer D / 2014 / Alternatives to antibiotic resistance marker genes for in vitro selection of genetically modified plants-scientific developments, current use, operational access and biosafety considerations / Crit Rev Plant Sci 33:286~330
Brookes G / 2014 / Economic impact of GM crops: the global income and production effects 1996-2012 / GM Crops Food 5:65~75
Chang YF / 1974 / d-lysine catabolic pathway in Pseudomonas putida: interrelations with l-lysine catabolism / J Bacteriol 117:753~764
Block DM / 1989 / Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants / Plant Physiol 91:694~701
Breyer D / 2014 / Alternatives to antibiotic resistance marker genes for in vitro selection of genetically modified plants-scientific developments, current use, operational access and biosafety considerations / Crit Rev Plant Sci 33:286~330
Brookes G / 2014 / Economic impact of GM crops: the global income and production effects 1996-2012 / GM Crops Food 5:65~75
Chang YF / 1974 / d-lysine catabolic pathway in Pseudomonas putida: interrelations with l-lysine catabolism / J Bacteriol 117:753~764
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