지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제46권 제5호
- 발행연도
- 2016.1
- 수록면
- 320 - 328 (9page)
이용수
초록· 키워드
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Purpose: Human saliva, as a vital part of the immune defense system, contains a number of distinct proteins and peptides. Recently human common salivary protein 1 (CSP1) has been identified as an abundant salivary protein and may play a role in promoting the binding of cariogenic bacteria to salivary pellicles. However, nothing else is known regarding the role of CSP1 in periodontology. The aim of this study was to quantify and compare CSP1 levels between healthy subjects and periodontal patients. Methods: This controlled clinical study was conducted in periodontally healthy individuals and patients with chronic periodontitis Chonbuk National University Hospital, with Institutional Review Board approval. Whole saliva samples were collected from 36 healthy subjects and 33 chronic periodontitis patients and analyzed. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis and immune blotting were conducted to ensure that anti-CSP1 monoclonal antibody (mAb) binds to CSP1 in human saliva. A sandwich enzymelinked immunosorbent assay (ELISA) system was house-fabricated using mAb-hCSP1#14 and mAb-hCSP1#4 as a capture and a detector mAb, respectively. The CSP1 concentrations in saliva from 36 healthy subjects and 33 periodontal patients were quantified using the CSP1 sandwich ELISA system, and the results were analyzed using the Student’s t -test. Results: Immunoblot analysis using mAb-hCSP1 as a probe confirmed that CSP1 in human saliva existed as a single band with a molecular weight of approximately 27-kDa. The quantification of CSP1 concentrations by CSP1 ELISA showed that the median values (25th to 75th percentiles) of periodontal patients and healthy subjects were 9,474 ng/mL (range, 8,434–10,139 ng/mL) and 8,598 ng/mL (range, 7,421–9,877 ng/mL), respectively. The Student’s t -test indicated the presence of a statistically significant difference between the 2 groups ( P =0.024). Conclusions: The presence of a significant difference in CSP1 levels between healthy subjects and periodontal patients suggests that CSP1 may be a potential biomarker for the detection or screening of periodontitis patients.
목차
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참고문헌 (20)
참고문헌 신청
Savage A / 2009 / A systematic review of definitions of periodontitis and methods that have been used to identify this disease / J Clin Periodontol 36:458~467
Baser U / 2014 / Is the severity of periodontitis related to gingival crevicular fluid and serum high-sensitivity C-reactive protein concentrations? / Clin Lab 60:1653~1658
Wilmarth PA / 2004 / Two-dimensional liquid chromatography study of the human whole saliva proteome / J Proteome Res 3:1017~1023
Yao Y / 2003 / Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches / J Biol Chem 278:5300~5308
Mullins JJ / 2006 / Identification of a human ortholog of the mouse Dcpp gene locus, encoding a novel member of the CSP-1/Dcpp salivary protein family / Physiol Genomics 28:129~140
Baser U / 2014 / Is the severity of periodontitis related to gingival crevicular fluid and serum high-sensitivity C-reactive protein concentrations? / Clin Lab 60:1653~1658
Wilmarth PA / 2004 / Two-dimensional liquid chromatography study of the human whole saliva proteome / J Proteome Res 3:1017~1023
Yao Y / 2003 / Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches / J Biol Chem 278:5300~5308
Mullins JJ / 2006 / Identification of a human ortholog of the mouse Dcpp gene locus, encoding a novel member of the CSP-1/Dcpp salivary protein family / Physiol Genomics 28:129~140
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