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Objective: To analyze the rheological and biochemical properties of saliva in healthy adults. Methods: The subjects were seven healthy young adults (20-39 years). The whole saliva was collected by the spittingmethod between 9:00 am and 12:00 am. Unstimulated saliva was collected first, which was followed by the collectionof stimulated saliva using citric acid was collected. The viscosity was measured using a capillary viscometer to examinethe rheological properties. The MUC5B concentration was analyzed using an enzyme-linked immunosorbentassay (ELISA) for the biochemical properties. Results: Ten and five minutes were required on average for unstimulated saliva and stimulated saliva, respectively, tocollect the minimum amount needed for analysis (8 ml). The flow rate increased from 1.037±0.323 ml/min to 1.823±0.660 ml/min after stimulation (P=0.018). Citric acid stimulation also increased the MUC5B concentration in the salivafrom 3.706±3.575 μg/ml to 6.928±2.03 μg/ml (P=0.028). The viscosity of saliva, however, did not show a significantdifference between with (1.1±0.164 cSt) or without (1.019±0.08 cSt) stimulation (P=0.128). A positive correlationwas observed between the flow rate and MUC5B concentration (Spearman’s rho=0.547, P=0.043). On the otherhand, there was no significant correlation between the MUC5B concentration and viscosity both in the unstimulatedand stimulated saliva (P=0.939 and 0.819, respectively). Conclusion: No significant correlation was observed between the MUC5B concentration and viscosity. Considering thetime required to collect samples, an analysis of the stimulated saliva appears feasible in various research settings. The feasibility of these analysis methods should be examined in a patient population.

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