지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제47권 제5호
- 발행연도
- 2017.1
- 수록면
- 292 - 311 (20page)
이용수
초록· 키워드
오류제보하기
Purpose: Beyond the limited scope of non-specific polyclonal regulatory T cell (Treg)-based immunotherapy, which depends largely on serendipity, the present study explored a target Treg subset appropriate for the delivery of a novel epitope spreader Pep19 antigen as part of a sophisticated form of immunotherapy with defined antigen specificity that induces immune tolerance.
Methods: Human polyclonal CD4+CD25+CD127lo− Tregs (127-Tregs) and naïve CD4+CD25+CD45RA+ Tregs (45RA-Tregs) were isolated and were stimulated with target peptide 19 (Pep19)-pulsed dendritic cells in a tolerogenic milieu followed by ex vivo expansion. Low-dose interleukin-2 (IL-2) and rapamycin were added to selectively exclude the outgrowth of contaminating effector T cells (Teffs). The following parameters were investigated in the expanded antigen-specific Tregs: the distinct expression of the immunosuppressive Treg marker Foxp3, epigenetic stability (demethylation in the Treg-specific demethylated region), the suppression of Teffs, expression of the homing receptors CD62L/CCR7, and CD95L-mediated apoptosis. The expanded Tregs were adoptively transferred into an NOD/scid/IL-2Rγ−/− mouse model of collagen-induced arthritis.
Results: Epitope-spreader Pep19 targeting by 45RA-Tregs led to an outstanding in vitro suppressive T cell fate characterized by robust ex vivo expansion, the salient expression of Foxp3, high epigenetic stability, enhanced T cell suppression, modest expression of CD62L/CCR7, and higher resistance to CD95L-mediated apoptosis. After adoptive transfer, the distinct fate of these T cells demonstrated a potent in vivo immunotherapeutic capability, as indicated by the complete elimination of footpad swelling, prolonged survival, minimal histopathological changes, and preferential localization of CD4+CD25+ Tregs at the articular joints in a mechanistic and orchestrated way.
Conclusions: We propose human naïve CD4+CD25+CD45RA+ Tregs and the epitope spreader Pep19 as cellular and molecular targets for a novel antigen-specific Treg-based vaccination against collagen-induced arthritis.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Ait-Oufella H / 2006 / Natural regulatory T cells control the development of atherosclerosis in mice / Nat Med 12 : 178 ~ 180
Arleevskaya MI / 2016 / How Rheumatoid arthritis can result from provocation of the immune system by microorganisms and viruses / Front Microbiol 7 : 1296 ~
Bluestone JA / 2012 / Current and future immunomodulation strategies to restore tolerance in autoimmune diseases / Cold Spring Harb Perspect Biol 4 : a007542 ~
Bluestone JA / 2015 / The therapeutic potential of regulatory T cells for the treatment of autoimmune disease / Expert Opin Ther Targets 19 : 1091 ~ 1103
Bluestone JA / 2015 / Type 1 diabetes immunotherapy using polyclonal regulatory T cells / Sci Transl Med 7 : 315ra189 ~
Arleevskaya MI / 2016 / How Rheumatoid arthritis can result from provocation of the immune system by microorganisms and viruses / Front Microbiol 7 : 1296 ~
Bluestone JA / 2012 / Current and future immunomodulation strategies to restore tolerance in autoimmune diseases / Cold Spring Harb Perspect Biol 4 : a007542 ~
Bluestone JA / 2015 / The therapeutic potential of regulatory T cells for the treatment of autoimmune disease / Expert Opin Ther Targets 19 : 1091 ~ 1103
Bluestone JA / 2015 / Type 1 diabetes immunotherapy using polyclonal regulatory T cells / Sci Transl Med 7 : 315ra189 ~
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Tumor-derived CD4+CD25+ Tregs Inhibit the Maturation and Antigen-Presenting Function of Dendritic Cells
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Upregulation of CD47 in Regulatory T Cells in Atopic Dermatitis
Yonsei Medical Journal
2016 .01
-
Extrinsic Acquisition of CD80 by Antigen-Specific CD8⁺ T Cells Regulates Their Recall Immune Responses to Acute Viral Infection
Immune Network
2019 .08
-
CD5 Expression Dynamically Changes During the Differentiation of Human CD8+ T Cells Predicting Clinical Response to Immunotherapy
Immune Network
2023 .08
-
Two Distinct Subsets Are Identified from the Peritoneal Myeloid Mononuclear Cells Expressing both CD11c and CD115
Immune Network
2019 .06
-
The Role of CD4 T Cell Help in CD8 T Cell Differentiation and Function During Chronic Infection and Cancer
Immune Network
2023 .10
-
Tumor Necrosis Factor and Regulatory T Cells
Yonsei Medical Journal
2019 .01
-
Effect of Peripheral Blood CD4 + CD25 + Regulatory T Cell on Postoperative Immunotherapy for Patients with Renal Carcinoma
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
T-Cells Modified with CD70 as an Alternative Cellular Vaccine for Antitumor Immunity
Cancer Research and Treatment
2020 .01
-
Development of Auto Antigen-specific Regulatory T Cells for Diabetes Immunotherapy
Immune Network
2016 .10
-
Attenuation of Peripheral Regulatory T-Cell Suppression of Skin-Homing CD8+T Cells in Atopic Dermatitis
Yonsei Medical Journal
2015 .01
-
Leukemia propagating cells in Philadelphia chromosome-positive ALL: a resistant phenotype with an adverse prognosis
Blood Research
2018 .01
-
The Roles of CCR7 for the Homing of Memory CD8+ T Cells into Their Survival Niches
Immune Network
2020 .06
-
Canine regulatory T cells expressing CD4+and CD25+ are proliferated by interleukin-2 cytokine
한국실험동물학회 학술발표대회 논문집
2018 .01
-
Effector Memory CD8+ and CD4+ T Cell Immunity Associated with Metabolic Syndrome in Obese Children
Pediatric Gastroenterology, Hepatology & Nutrition
2021 .01
-
Effect of IL-4 on the Development and Function of Memory-like CD8 T Cells in the Peripheral Lymphoid Tissues
Immune Network
2016 .04
-
CD9 Expression in Colorectal Carcinomas and Its Prognostic Significance
Journal of Pathology and Translational Medicine
2016 .01
-
CD80CD86 deficiency disrupts regulator T cell homeostasis and induces autoimmune alopecia
한국실험동물학회 학술발표대회 논문집
2016 .08
-
Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn’s disease
Intestinal research
2017 .01
-
Prognostic Significance of CD109 Expression in Patients with Ovarian Epithelial Cancer
Journal of Pathology and Translational Medicine
2019 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0