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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 138 - 146 (9page)
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Background : We developed and evaluated the utility of a multiplex real-time PCR assay that uses melting curve analysis and allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci.
Methods : The specificity of the assay was tested using 4 reference strains of vancomycin-resistant enterococci (VRE) and 2 reference strains of vancomycin-susceptible enterococci. Ninety-three clinical isolates of enterococci with different glycopeptide-resistant phenotypes were genotyped and identified using a multiplex real-time PCR assay and melting curve analysis.
Results : Representative melting curves were obtained for Enterococcus faecium, Enterococcus faecalis, vanA-containing E. faecium, vanB-containing E. faecalis, Enterococcus gallinarum, and Enterococcus casseliflavus. Phenotypic and genotypic analysis of the isolates revealed same results for 82 enterococcal isolates, while in 4 isolates, the glycopeptide-resistant phenotypes were inconsistent with the glycopeptide-resistant genotypes and in the 4 other isolates, species could not be accurately identified. Three isolates with mixed strains, which were detected by the PCR assay, could not be correctly identified using phenotypic methods.
Conclusions : VRE genotyping and identification of clinically relevant enterococci were rapidly and correctly performed using multiplex real-time PCR assay and melting curve analysis. (Korean J Lab Med 2010;30:138-46)
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참고문헌 (27)
참고문헌 신청
Boyd DA / 2008 / Molecular characterization of Enterococcus faecalis N06-0364 with low-level vancomycin resistance harboring a novel D-Ala-D-Ser gene cluster, vanL / Antimic
Chang S / 2003 / Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene / N Engl J Med 348 : 1342 ~ 1347
Clark NC / 1993 / Characterization of glycopeptide-resistant enterococci from U.S. hospitals / Antimicrob Agents Chemother 37 : 2311 ~ 2317
Clinical and Laboratory Standards Institute / 2009 / Performance standards for antimicrobial susceptibility testing: nineteenth informational supplement / Clinical and Laboratory Standa
Dutka-Malen S / 1995 / Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR / J Clin Microbiol 33
Chang S / 2003 / Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene / N Engl J Med 348 : 1342 ~ 1347
Clark NC / 1993 / Characterization of glycopeptide-resistant enterococci from U.S. hospitals / Antimicrob Agents Chemother 37 : 2311 ~ 2317
Clinical and Laboratory Standards Institute / 2009 / Performance standards for antimicrobial susceptibility testing: nineteenth informational supplement / Clinical and Laboratory Standa
Dutka-Malen S / 1995 / Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR / J Clin Microbiol 33
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