지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 326 - 330 (5page)
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Background: Active screening for vancomycin-resistant enterococci (VRE) using rectal specimens is recommended to limit the spread of antimicrobial resistance within certain high-risk populations. We evaluated the diagnostic performance of Vancomycin Resistance 3 Multiplexed Tandem PCR assay (AusDiagnostics, Australia), a rapid multiplex realtime PCR assay that detects vanA and/or vanB.
Methods: Two-hundred-and-eleven rectal swabs from Hematology and Oncology unit were submitted for VRE surveillance via direct detection of vanA and/or vanB by culture and by using Vancomycin Resistance 3 Multiplexed Tandem PCR assay. Enterococci were identified to the species level by using standard biochemical tests and BD Phoenix Automated Microbiology System (BD Diagnostic Systems, USA). Vancomycin susceptibility of enterococci was determined using Etest (BioMerieux, France).
Results: Compared to the culture method, Vancomycin Resistance 3 Multiplexed Tandem PCR assay had a sensitivity of 84.0%, specificity of 98.8%, positive predictive value (PPV)of 91.3%, and negative predictive value (NPV) of 97.6%. The assay failed to detect 18(8.5%) specimens because of the presence of PCR inhibitors; of the remaining 193 specimens,25 (12.9%) were positive, 23 for vanA, and 2 for vanB. Although both sensitivity and specificity for vanA VRE was 100% compared to the culture method, all vanB-positive specimens tested negative by VRE culture.
Conclusions: Vancomycin Resistance 3 Multiplexed Tandem PCR assay is a rapid and laborsaving option for VRE surveillance for direct use on rectal swabs. However, the high rate of PCR failure owing to the inhibitors in the specimens and the low specificity for vanB should be considered when interpreting the results.
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참고문헌 (21)
참고문헌 신청
[학술지(정기간행
Ballard SA / 2005 / Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci:
Brown DF / 2008 / Non-susceptibility trends among enterococci and non-pneumococcal streptococci from bacteraemias in the UK and Ireland, 2001 to 2006 / J Antimicrob Chemothe
Colak D / 2002 / First outbreak of vancomycin-resistant enterococci in a tertiary hospital in Turkey / J Antimicrob Chemother 50 : 397 ~ 401
Courvalin P / 2006 / Vancomycin resistance in gram-positive cocci / Clin Infect Dis 42 (S1) : S25 ~ S34
Ballard SA / 2005 / Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci:
Brown DF / 2008 / Non-susceptibility trends among enterococci and non-pneumococcal streptococci from bacteraemias in the UK and Ireland, 2001 to 2006 / J Antimicrob Chemothe
Colak D / 2002 / First outbreak of vancomycin-resistant enterococci in a tertiary hospital in Turkey / J Antimicrob Chemother 50 : 397 ~ 401
Courvalin P / 2006 / Vancomycin resistance in gram-positive cocci / Clin Infect Dis 42 (S1) : S25 ~ S34
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