지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1,229 - 1,242 (14page)
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초록· 키워드
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Purpose We developed a new method of detecting circulating tumor cells (CTCs) in liver cancer patients by constructing cell blocks from peripheral blood cells, including CTCs, followed by multiple immunohistochemical analysis.
Materials and Methods Cell blocks were constructed from the nucleated cell pellets of peripheral blood after removal of red blood cells. The blood cell blocks were obtained from 29 patients with liver cancer, and from healthy donor blood spiked with seven cell lines. The cell blocks and corresponding tumor tissues were immunostained with antibodies to seven markers: cytokeratin (CK), epithelial cell adhesion molecule (EpCAM), epithelial membrane antigen (EMA), CK18, -fetoprotein (AFP), Glypican 3, and HepPar1.
Results The average recovery rate of spiked SW620 cells from blood cell blocks was 91%. CTCs were detected in 14 out of 29 patients (48.3%); 11/23 hepatocellular carcinomas (HCC), 1/2 cholangiocarcinomas (CC), 1/1 combined HCC-CC, and 1/3 metastatic cancers. CTCs from 14 patients were positive for EpCAM (57.1%), EMA (42.9%), AFP (21.4%), CK18 (14.3%), Gypican3 and CK (7.1%, each), and HepPar1 (0%). Patients with HCC expressed EpCAM, EMA, CK18, and AFP in tissue and/or CTCs, whereas CK, HepPar1, and Glypican3 were expressed only in tissue. Only EMA was significantly associated with the expressions in CTC and tissue. CTC detection was associated with higher T stage and portal vein invasion in HCC patients.
Conclusion This cell block method allows cytologic detection and multiple immunohistochemical analysis of CTCs. Our results show that tissue biomarkers of HCC may not be useful for the detection of CTC. EpCAM could be a candidate marker for CTCs in patients with HCC.
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참고문헌 (29)
참고문헌 신청
Jemal A / 2011 / Global cancer statistics / CA Cancer J Clin 61:69~90
Park JW / 2015 / Global patterns of hepatocellular carcinoma management from diagnosis to death : the BRIDGE Study / Liver Int 35:2155~2166
Arnaoutakis DJ / 2014 / Recurrence patterns and prognostic factors in patients with hepatocellular carcinoma in noncirrhotic liver : a multi-institutional analysis / Ann Surg Oncol 21:147~154
Sumie S / 2014 / The significance of classifying microvascular invasion in patients with hepatocellular carcinoma / Ann Surg Oncol 21:1002~1009
Harouaka R / 2014 / Circulating tumor cells : advances in isolation and analysis, and challenges for clinical applications / Pharmacol Ther 141:209~221
Park JW / 2015 / Global patterns of hepatocellular carcinoma management from diagnosis to death : the BRIDGE Study / Liver Int 35:2155~2166
Arnaoutakis DJ / 2014 / Recurrence patterns and prognostic factors in patients with hepatocellular carcinoma in noncirrhotic liver : a multi-institutional analysis / Ann Surg Oncol 21:147~154
Sumie S / 2014 / The significance of classifying microvascular invasion in patients with hepatocellular carcinoma / Ann Surg Oncol 21:1002~1009
Harouaka R / 2014 / Circulating tumor cells : advances in isolation and analysis, and challenges for clinical applications / Pharmacol Ther 141:209~221
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