지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 297 - 304 (8page)
- DOI
- 10.47429/lmo.2021.11.4.297
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초록· 키워드
오류제보하기
Background: Circulating tumor cells (CTCs) are rare in the circulation of patients with tumors and are very difficult to detect. In this study, we cultured CTCs in a three-dimensionally reprogrammed condition using the peripheral blood of patients with cancer. Methods: We evaluated the sensitivity of a three-dimensional (3D) conditionally reprogrammed cell (CRC) culture method using SNU-484 gastric cancer cells. From April 2017 to January 2018, blood was collected from 30 patients diagnosed with colorectal or breast cancer at the Daegu Catholic University Medical Center. After lysis of the red blood cells, the blood samples were centrifuged to isolate the mononuclear cells. We used Matrigel to create 3D conditions and reprogrammed the isolated cells using a Rho-kinase inhibitor and J2 feeder cells. After 30 days of culture, the cells were collected and RNA was extracted and amplified for the expression of GAPD, hTERT, and MAGE A1-6 using real-time PCR. Results: The SNU-484 cells were cultured from three cells using the 3D CRC culture method. The cultured blood samples showed some cells or few colonies. The positive rates of hTERT and MAGE A1-6 mRNA were 50% and 30%, respectively. The positive rates of both mRNAs were 60% and 20%, respectively. MAGE and both mRNAs showed significantly increased odds ratios at the tumor, nodes, and metastases (TNM), N, and M stages in all subjects. Conclusions: As 60% of CTCs were detected in the blood of 30 patients with cancer, the 3D CRC culture method was very effective for CTC cul ture.
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등록된 정보가 없습니다.
참고문헌 (34)
참고문헌 신청
Ashworth TR / 1869 / A case of cancer in which cells similar to those in the tumours were seen in the blood after death / Aust Med J 14:146~147
Pecot CV / 2011 / A novel platform for detection of CK+ and CK-CTCs / Cancer Discov 1:580~586
Marrinucci D / 2012 / Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers / Phys Biol 9:016003
Haber DA / 2014 / Blood-based analyses of cancer : circulating tumor cells and circulating tumor DNA / Cancer Discov 4:650~661
Azim HA, Jr. / 2013 / Circulating tumor cells and response to neoadjuvant paclitaxel and HER2-targeted therapy: a sub-study from the NeoALTTO phase III trial / Breast 22:1060~1065
Pecot CV / 2011 / A novel platform for detection of CK+ and CK-CTCs / Cancer Discov 1:580~586
Marrinucci D / 2012 / Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers / Phys Biol 9:016003
Haber DA / 2014 / Blood-based analyses of cancer : circulating tumor cells and circulating tumor DNA / Cancer Discov 4:650~661
Azim HA, Jr. / 2013 / Circulating tumor cells and response to neoadjuvant paclitaxel and HER2-targeted therapy: a sub-study from the NeoALTTO phase III trial / Breast 22:1060~1065
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