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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 37 - 42 (6page)
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The attachment and adhesion of RAW 264.7 and MC3T3- E1 cells to titanium (Ti) discs with various degrees of roughness was investigated. The attachment, adhesion, and proliferation of these cells were evaluated after 4 hr, 24 hr and 7 day incubations. Both RAW 264.7 and MC3T3-E1 cells showed a time-dependant correlation between attachment and adhesion on the surface of the titanium discs. Both types of cells tended to have higher survival rate on these discs as the surface roughness increased. The percentage of adherent inflammatory RAW 264.7 cells was greater than MC3T3-E1 cells at 24 hr, but this was reversed at 7 days in culture. The morphology of osteoblastic MC3T3-E1 cells at 24 hr, determined using a surface emission microscope (SEM), appeared flattened and spread out while inflammatory RAW 264.7 cells were predominantly spherical in shape. The adhesion of both cell types on the titanium discs was dependant on the levels of fibronectin adsorbed on the disc surface, indicating that serum constituents modulate the efficient adhesion of these cells. Our data indicate that the cellular response to the titanium surface is dependent on the types of cells, surface roughness and serum constituents.
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참고문헌 (22)
참고문헌 신청
Anselme K / 2000 / Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses / J Biomed Mater Res 49 : 155 ~ 166
Baek JH / 2002 / Interactions between fibronectin and osteoblast are important in osteoblast differentiation and mineralized nodule formation / Int J Oral Biol 27 : 61 ~ 67
Degasne I / 1999 / Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2) on titanium surfaces
Duewelhenke N / 2007 / Serum-free cultivation of osteoprogenitor cells and osteoblasts to test biomaterials / Orthopade 36 : 220 ~ 226
Galli C / 2005 / Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces / J Periodontol 76 (3) : 364 ~ 372
Baek JH / 2002 / Interactions between fibronectin and osteoblast are important in osteoblast differentiation and mineralized nodule formation / Int J Oral Biol 27 : 61 ~ 67
Degasne I / 1999 / Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2) on titanium surfaces
Duewelhenke N / 2007 / Serum-free cultivation of osteoprogenitor cells and osteoblasts to test biomaterials / Orthopade 36 : 220 ~ 226
Galli C / 2005 / Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces / J Periodontol 76 (3) : 364 ~ 372
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