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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치주과학회 Journal of Periodontal & Implant Science Journal of Periodontal & Implant Science 제38권 제2호
- 발행연도
- 2008.1
- 수록면
- 299 - 308 (10page)
이용수
초록· 키워드
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Purpose: The aim of this study was to evaluate adhesion and gene expression of the MC3T3-E1 cells cultured on machined
titanium surface (MS) and anodized titanium surface (AS) using MTT test, Scanning electron micrograph and cDNA microarray.
Materials and Methods: The MTT test assay was used for examining the proliferation of MC3T3-E1 cells, osteoblast like cells
from Rat calvaria, on MS and AS for 24 hours and 48 hours. Cell cultures were incubated for 24 hours to evaluate the
influence of the substrate geometry on both surfaces using a Scanning Electron Micrograph (SEM). The cDNA microarray
Agilent Rat 22K chip was used to monitor expressions of genes.
Results: After 24 hours of adhesion, the cell density on AS was higher than MS (p < 0.05). After 48 hours the cell density on
both titanium surfaces were similar (p > 0.05). AS had the irregular, rough and porous surface texture. After 48 hours incubation
of the MC3T3-E1 cells, connective tissue growth factor (CTGF) was up-regulated on AS than MS (more than 2 fold) and the
insulin-like growth factor 1 receptor was down-regulated (more than 2 fold) on AS than MS.
Conclusion: Microarray assay at 48 hours after culturing the cells on both surfaces revealed that osteoinductive molecules
appeared more prominent on AS, whereas the adhesion molecules on the biomaterial were higher on MS than AS, which will
affect the phenotype of the plated cells depending on the surface morphology. (J Korean Acad Periodontol 2008;38:299-308)
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Albrektsson T / 2004 / Oral implant surfaces: Part 2--review focusing on clinical knowledge of different surfaces / Int J Prosthodont 17 : 544 ~ 564
Carinci F / 2003 / Analysis of osteoblast-like MG63 cells? response to a rough implant surface by means of DNA microarray / J Oral Implantol 29 : 215 ~ 220
Carinci F / 2003 / Titanium-Cell Interaction: Analysis of Gene Expression Profiling / J Biomed Mater Res B Appl Biomater 66 : 341 ~ 346
Carinci F / 2004 / Analysis of MG63 osteoblastic-cell response to a new nanoporous implant surface by means of a microarray technology / Clin Oral Impl Res 15 : 180 ~ 186
Carinci F / 2004 / Zirconium oxide: analysis of MG63 osteoblast-like cell response by means of a microarray technology / Biomaterials 25 : 215 ~ 218
Carinci F / 2003 / Analysis of osteoblast-like MG63 cells? response to a rough implant surface by means of DNA microarray / J Oral Implantol 29 : 215 ~ 220
Carinci F / 2003 / Titanium-Cell Interaction: Analysis of Gene Expression Profiling / J Biomed Mater Res B Appl Biomater 66 : 341 ~ 346
Carinci F / 2004 / Analysis of MG63 osteoblastic-cell response to a new nanoporous implant surface by means of a microarray technology / Clin Oral Impl Res 15 : 180 ~ 186
Carinci F / 2004 / Zirconium oxide: analysis of MG63 osteoblast-like cell response by means of a microarray technology / Biomaterials 25 : 215 ~ 218
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