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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한치과보철학회 The Journal of Advanced of Prosthodontics The Journal of Advanced of Prosthodontics 제7권 제2호
- 발행연도
- 2015.1
- 수록면
- 138 - 145 (8page)
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PURPOSE. The objective of this study was to conduct an in vitro comparative evaluation of polished and laserdimpled titanium (Ti) surfaces to determine whether either surface has an advantage in promoting the attachment of epithelial-like cells and fibroblast to Ti.
MATERIALS AND METHODS. Forty-eight coin-shaped samples of commercially pure, grade 4 Ti plates were used in this study. These discs were cleaned to a surface roughness (Ra: roughness centerline average) of 180 nm by polishing and were divided into three groups: SM (n=16) had no dimples and served as the control, SM15 (n=16) had 5-µm dimples at 10-µm intervals, and SM30 (n=16) had 5-µm dimples at 25-µm intervals in a 2 × 4 mm2 area at the center of the disc. Human gingival squamous cell carcinoma cells (YD-38) and human lung fibroblasts (MRC-5) were cultured and used in cell proliferation assays, adhesion assays, immunofluorescent staining of adhesion proteins, and morphological analysis by SEM. The data were analyzed statistically to determine the significance of differences. RESULTS. The adhesion strength of epithelial cells was higher on Ti surfaces with 5-µm laser dimples than on polished Ti surfaces, while the adhesion of fibroblasts was not significantly changed by laser treatment of implant surfaces. However, epithelial cells and fibroblasts around the laser dimples appeared larger and showed increased expression of adhesion proteins. CONCLUSION. These findings demonstrate that laser dimpling may contribute to improving the periimplant soft tissue barrier. This study provided helpful information for developing the transmucosal surface of the abutment.
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참고문헌 (24)
참고문헌 신청
Abrahamsson I / 1996 / The peri-implant hard and soft tissues at different implant systems. A comparative study in the dog / Clin Oral Implants Res 7 : 212 ~ 219
Abrahamsson I / 2002 / The mucosal attachment to titanium implants with different surface characteristics: an experimental study in dogs / J Clin Periodontol 29 : 448 ~ 455
An N / 2012 / Initial attachment, subsequent cell proliferation/viability and gene expression of epithelial cells related to attachment and wound healing in response to diff
Baharloo B / 2005 / Substratum roughness alters the growth, area, and focal adhesions of epithelial cells, and their proximity to titanium surfaces / J Biomed Mater Res A 74
Berglundh T / 1996 / Dimension of the periimplant mucosa. Biological width revisited / J Clin Periodontol 23 : 971 ~ 973
Abrahamsson I / 2002 / The mucosal attachment to titanium implants with different surface characteristics: an experimental study in dogs / J Clin Periodontol 29 : 448 ~ 455
An N / 2012 / Initial attachment, subsequent cell proliferation/viability and gene expression of epithelial cells related to attachment and wound healing in response to diff
Baharloo B / 2005 / Substratum roughness alters the growth, area, and focal adhesions of epithelial cells, and their proximity to titanium surfaces / J Biomed Mater Res A 74
Berglundh T / 1996 / Dimension of the periimplant mucosa. Biological width revisited / J Clin Periodontol 23 : 971 ~ 973
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