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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 55 - 62 (8page)
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In the present study in vitro mutagenesis was used to study the effect of gamma irradiation and EMS on callus induction, morphogenesis and production of multiple shoots from different explants of Citrullus colocynthis (L.) Schrad. Gamma radiations (5 kR to 20 kR) and certain chemicals have been effected on plant growth developments and changes of biochemical metabolisms in plants. Murashige and Skoog (MS) medium containing with auxins such as NAA, IAA, 2,4-D (0.5 ~ 2.0 mg/l), cytokinines BAP, kn TDZ, (0.5 ~ 2.5 mg/l), L-Glutamic acid (1 ~ 2 mg/l) and Coconut milk (10 ~ 20%). After 5 weeks on induction media, explants and callus (EC) were exposed to 5 kR, 10 kR, 15 kR and 20kR, of gamma radiation and treated with 1, 2, 3, 4 and 5 mM ethyl methane sulphonate (EMS) for 30 min. The highest percentage of callusing was observed (70%) stem irradiated with 5 kR and significantly decrease in fresh and dry weight of callus in the below 4 kR doses and above 20 kR doses, there was a progressive decrease in the fresh weight and dry weights when compared to control callus. Maximum percentage of plantlet regeneration (59%) was induced from callus exposed to 15 kR gamma irradiation on MS media fortified with 2.0 mg/l 2,4-D + 2.0 mg/l BAP + 2.0 mg/l L-glutamic acid. Increase in gamma irradiation dose above 15 kR and 5 mM EMS reduced regeneration capacity of callus. Doses higher than 20 kR and 7 mM EMS was lethal to micropropagated plants of Citurullus colocynthis.
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참고문헌 (43)
참고문헌 신청
Arya HC / 1969 / Effect of gamma irradiation on callus growth of Phyllonera gall and normal grape stem cells in tissue / Can J Bot 47:1623~1628
Bajaj YPS / 1970 / Effect of gamma radiation on growth, RNA protein and nitrogen contents of bean callus cultures / Ann Bot 34:1084~1096
Bassam AL / 1990 / The effect of gamma irradiation on the growth of cytology of carrot (Daucus carota L.) tissue culture / Envr and Exptl Bot 30(3):361~371
Bauman H / 2001 / Theor. Appl Gen 102:111~117
Broerties C / 1976 / Ornamental crops. Development in crop science (2): Application of Mutation Breeding Methods in the Improvement of vegetatively Propagated Crops / Elsevier Scientific Publishing Company :76
Bajaj YPS / 1970 / Effect of gamma radiation on growth, RNA protein and nitrogen contents of bean callus cultures / Ann Bot 34:1084~1096
Bassam AL / 1990 / The effect of gamma irradiation on the growth of cytology of carrot (Daucus carota L.) tissue culture / Envr and Exptl Bot 30(3):361~371
Bauman H / 2001 / Theor. Appl Gen 102:111~117
Broerties C / 1976 / Ornamental crops. Development in crop science (2): Application of Mutation Breeding Methods in the Improvement of vegetatively Propagated Crops / Elsevier Scientific Publishing Company :76
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