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논문 기본 정보

자료유형
학술저널
저자정보
Rahman Zuraida Abd (Biotechnology & Nanotechnology Research Centre) Seman Zulkifli Ahmad (Biotechnology & Nanotechnology Research Centre) Othman Ayu Nazreena (Biotechnology & Nanotechnology Research Centre) Ghaffar Mohamad Bahagia Ab (Research Innovation Centre Excellence) Razak Shahril Ab (Centre for Marker Discovery and Validation) Yusof Muhammad Fairuz Mohd (Centre for Marker Discovery and Validation) Nasir Khairun Hisam (Centre for Marker Discovery and Validation) Ahmad Khairulmazmi (Universiti Putra Malaysia) Chow Yeow Lit (School of Biological Science Universiti Sains Mal) How Teo Chee (Universiti Malaya) Saad Norsharina Md (Universiti Malaya) Subramaniam Sreeramanan (Universiti Malaya)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제3호
발행연도
2022.6
수록면
343 - 355 (13page)
DOI
10.1007/s11816-022-00742-4

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The current study recognised the issues encountered in regenerating Malaysia MR219 rice plantlet via microspore culture and attempted to develop an efficient protocol in overcoming the restraints. In the present study, a high proportion of uninu- cleate microspores (49.17%) was isolated from Stage 2-Segment II panicle (59?61?days), which also exhibited the highest callus initiation rate of 8.50%. Maintenance of the panicles under a cool temperature of 4?°C for 7?days before isolating the microspores, resulted in the highest microspore viability of 58.33% and callus initiation rate of 9.33%. The microspore isola- tion protocol was also optimised in the present study. The filtration sieve engagement with a pore size of 80?μm and further suspension centrifugation at 800?rpm for 5?min produced the highest microspore viability percentage and callus initiation rate. The incorporation of 3.0?mg/L kinetin in conjunction with 0.5?mg/L 2,4-D greatly enhanced the callus initiation rate, with 11.33%. The callus proliferation capacity, with the formation of 481.67?mg callus, was significantly promoted by the addition of 1.0?mg/L kinetin and 0.5?mg/L 2,4-D into the growth medium. Moreover, a higher green plantlet regeneration frequency of 2.83% was induced by the supplementation of 8% sucrose, which produced an average of 3.50 green plantlets.

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