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논문 기본 정보

자료유형
학술저널
저자정보
Hong-Rui Ning (Anhui Agricultural University) Si-Yang Huang (Chinese Academy of Agricultural Sciences) Jin-Lei Wang (Chinese Academy of Agricultural Sciences) Qian-Ming Xu (Anhui Agricultural University) Xing-Quan Zhu (Anhui Agricultural University)
저널정보
대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.3
발행연도
2015.6
수록면
345 - 348 (4page)

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Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.

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UCI(KEPA) : I410-ECN-0101-2016-513-001617906