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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 29 - 37 (9page)
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Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesd-the major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs).
Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity.
Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells.
Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.
Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity.
Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells.
Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (26)
참고문헌 신청
1. [학술지(정기간행물)] - Kiefer D / 2003 / Panax ginseng / Am Fam Physician 68 : 1539 ~ 1542
2. [학술지(정기간행물)] - Lee TK / 2005 / Radioprotective potential of ginseng / Mutagenesis 20 : 237 ~ 243
3. [학술지(정기간행물)] - Kang KA / 2008 / Ginseng total saponin enhances the phagocytic capacity of canine peripheral blood phagocytes in vitro / Am J Chin Med 36 : 329 ~ 341
4. [학술지(정기간행물)] - Fan ZH / 1995 / Enhancement of nitric oxide production from activated macrophages by a purified form of ginsenoside (Rg1) / Am J Chin Med 23
5. [학술지(정기간행물)] - Park EK / 2004 / Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities / Int Arch Allergy Immunol 133 : 113 ~ 120
2. [학술지(정기간행물)] - Lee TK / 2005 / Radioprotective potential of ginseng / Mutagenesis 20 : 237 ~ 243
3. [학술지(정기간행물)] - Kang KA / 2008 / Ginseng total saponin enhances the phagocytic capacity of canine peripheral blood phagocytes in vitro / Am J Chin Med 36 : 329 ~ 341
4. [학술지(정기간행물)] - Fan ZH / 1995 / Enhancement of nitric oxide production from activated macrophages by a purified form of ginsenoside (Rg1) / Am J Chin Med 23
5. [학술지(정기간행물)] - Park EK / 2004 / Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities / Int Arch Allergy Immunol 133 : 113 ~ 120
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UCI(KEPA) : I410-ECN-0101-2018-524-001597535