지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 298 - 306 (9page)
이용수
초록· 키워드
오류제보하기
Background: Compound K (CK) is a bioactive derivative of ginsenoside Rb1 in Panax ginseng (Korean ginseng). Its biological and pharmacological activities have been studied in various disease conditions, although its immunomodulatory role in innate immunity mediated by monocytes/macrophages has been poorly understood. In this study, we aimed to elucidate the regulatory role of CK on cellular events mediated by monocytes and macrophages in innate immune responses.
Methods: The immunomodulatory role of CK was explored by various immunoassays including cell-cell adhesion, fibronectin adhesion, cell migration, phagocytic uptake, costimulatory molecules, reactive oxygen species production, luciferase activity, and by the measurement of mRNA levels of proinflammatory genes.
Results: Compound K induced cell cluster formation through cell-cell adhesion, cell migration, and phagocytic activity, but it suppressed cell-tissue interactions in U937 and RAW264.7 cells. Compound K also upregulated the surface expression of the cell adhesion molecule cluster of differentiation (CD) 43 (CD43) and costimulatory molecules CD69, CD80, and CD86, but it downregulated the expression of monocyte differentiation marker CD82 in RAW264.7 cells. Moreover, CK induced the release of reactive oxygen species and induced messenger RNA expression of proinflammatory genes, inducible nitric oxide synthase, and tumor necrosis factor-alpha by enhancing the nuclear translocation and transcriptional activities of nuclear factor kappa-B and activator protein-1.
Conclusion: Our results suggest that CK has an immunomodulatory role in innate immune responses through regulating various cellular events mediated by monocytes and macrophages.
Methods: The immunomodulatory role of CK was explored by various immunoassays including cell-cell adhesion, fibronectin adhesion, cell migration, phagocytic uptake, costimulatory molecules, reactive oxygen species production, luciferase activity, and by the measurement of mRNA levels of proinflammatory genes.
Results: Compound K induced cell cluster formation through cell-cell adhesion, cell migration, and phagocytic activity, but it suppressed cell-tissue interactions in U937 and RAW264.7 cells. Compound K also upregulated the surface expression of the cell adhesion molecule cluster of differentiation (CD) 43 (CD43) and costimulatory molecules CD69, CD80, and CD86, but it downregulated the expression of monocyte differentiation marker CD82 in RAW264.7 cells. Moreover, CK induced the release of reactive oxygen species and induced messenger RNA expression of proinflammatory genes, inducible nitric oxide synthase, and tumor necrosis factor-alpha by enhancing the nuclear translocation and transcriptional activities of nuclear factor kappa-B and activator protein-1.
Conclusion: Our results suggest that CK has an immunomodulatory role in innate immune responses through regulating various cellular events mediated by monocytes and macrophages.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and Discussion
References
참고문헌 (46)
참고문헌 신청
[학술지(정기간행물)] - Lee YG / 2011 / Functional role of Akt in macrophage-mediated innate immunity / Front Biosci (Landmark Ed) 16 : 517 ~ 530
[학술지(정기간행물)] - Yu T / 2012 / The pivotal role of TBK1 in inflammatory responses mediated by macrophages / Mediators Inflamm 2012 : 979105 ~
[학술지(정기간행물)] - Byeon SE / 2012 / The role of Src kinase in macrophage-mediated inflammatory responses / Mediators Inflamm 2012 : 512926 ~
[학술지(정기간행물)] - Yi YS / 2014 / Functional roles of Syk in macrophage-mediated inflammatory responses / Mediators Inflamm 2014 : 270302 ~
[학술지(정기간행물)] - Yang Y / 2014 / Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses / Mediators Inflamm 2014 : 352371 ~
[학술지(정기간행물)] - Yu T / 2012 / The pivotal role of TBK1 in inflammatory responses mediated by macrophages / Mediators Inflamm 2012 : 979105 ~
[학술지(정기간행물)] - Byeon SE / 2012 / The role of Src kinase in macrophage-mediated inflammatory responses / Mediators Inflamm 2012 : 512926 ~
[학술지(정기간행물)] - Yi YS / 2014 / Functional roles of Syk in macrophage-mediated inflammatory responses / Mediators Inflamm 2014 : 270302 ~
[학술지(정기간행물)] - Yang Y / 2014 / Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses / Mediators Inflamm 2014 : 352371 ~
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UCI(KEPA) : I410-ECN-0101-2018-524-001598996