지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 322 - 330 (9page)
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초록· 키워드
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Background: UV-irradiated keratinocytes secrete various proinflammatory cytokines. UV-induced skin damage is mediated by growth factors and proinflammatory cytokines such as granulocyte macrophage colony stimulating factor (GM-CSF). In a previous study, we found that the saponin of Korean Red Ginseng (SKRG) decreased the expression of GM-CSF in UVB-irradiated SP-1 keratinocytes. In this study, we attempted to find the inhibitory mechanism of SKRG on UVB-induced GM-CSF expression in SP-1 keratinocytes.
Methods: We investigated the inhibitory mechanism of SKRG and ginsenosides from Panax ginseng on UVB-induced GM-CSF expression in SP-1 keratinocytes.
Results: Treatment with SKRG decreased the expression of GM-CSF mRNA and protein induced by irradiation of UVB in SP-1 keratinocytes. The phosphorylation of ERK was induced by UVB at 10 min, and decreased with SKRG treatment in SP-1 keratinocytes. In addition, treatment with SKRG inhibited the UVB-induced phosphorylation of epidermal growth factor receptor (EGFR), which is known to be an upstream signal of ERK. From these results, we found that the inhibition of GM-CSF expression by SKRG was derived from the decreased phosphorylation of EGFR. To identify the specific compound composing SKRG, we tested fifteen kinds of ginsenosides. Among these compounds, ginsenoside-Rh3 decreased the expression of GM-CSF protein and mRNA in SP-1 keratinocytes.
Conclusion: Taken together, we found that treatment with SKRG decreased the phosphorylation of EGFR and ERK in UVB-irradiated SP-1 keratinocytes and subsequently inhibited the expression of GM-CSF. Furthermore, we identified ginsenoside-Rh3 as the active saponin in Korean Red Ginseng.
Methods: We investigated the inhibitory mechanism of SKRG and ginsenosides from Panax ginseng on UVB-induced GM-CSF expression in SP-1 keratinocytes.
Results: Treatment with SKRG decreased the expression of GM-CSF mRNA and protein induced by irradiation of UVB in SP-1 keratinocytes. The phosphorylation of ERK was induced by UVB at 10 min, and decreased with SKRG treatment in SP-1 keratinocytes. In addition, treatment with SKRG inhibited the UVB-induced phosphorylation of epidermal growth factor receptor (EGFR), which is known to be an upstream signal of ERK. From these results, we found that the inhibition of GM-CSF expression by SKRG was derived from the decreased phosphorylation of EGFR. To identify the specific compound composing SKRG, we tested fifteen kinds of ginsenosides. Among these compounds, ginsenoside-Rh3 decreased the expression of GM-CSF protein and mRNA in SP-1 keratinocytes.
Conclusion: Taken together, we found that treatment with SKRG decreased the phosphorylation of EGFR and ERK in UVB-irradiated SP-1 keratinocytes and subsequently inhibited the expression of GM-CSF. Furthermore, we identified ginsenoside-Rh3 as the active saponin in Korean Red Ginseng.
목차
abstract
1. Introduction
2. Materials and methods
3. Results
4. Discussion
References
참고문헌 (29)
참고문헌 신청
[학술지(정기간행물)] - Han W / 2009 / Requirement for metalloproteinase-dependent ERK and AKT activation in UVB-induced G1-S cell cycle progression of human keratinocytes / Photochem Photobiol 85 : 997 ~ 1003
[학술지(정기간행물)] - Singh B / 2009 / UV-induced EGFR signal transactivation is dependent on proligand shedding by activated metalloproteases in skin cancer cell lines / Int J Cancer 124 : 531 ~ 539
[학술지(정기간행물)] - Lee EJ / 2010 / Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling / Free Radical Bio Med 48 : 1133 ~ 1143
[학술지(정기간행물)] - Hamilton JA / 2008 / Colony-stimulating factors in inflammation and autoimmunity / Nat Rev Immunol 8 : 533 ~ 544
[학술지(정기간행물)] - Kanda N / 2004 / Histamine enhances the production of granulocytemacrophage colony-stimulating factor via protein kinase C alpha and extracellular signal-regulated kinase in human keratinocytes / J Invest Dermatol 122 : 863 ~ 872
[학술지(정기간행물)] - Singh B / 2009 / UV-induced EGFR signal transactivation is dependent on proligand shedding by activated metalloproteases in skin cancer cell lines / Int J Cancer 124 : 531 ~ 539
[학술지(정기간행물)] - Lee EJ / 2010 / Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling / Free Radical Bio Med 48 : 1133 ~ 1143
[학술지(정기간행물)] - Hamilton JA / 2008 / Colony-stimulating factors in inflammation and autoimmunity / Nat Rev Immunol 8 : 533 ~ 544
[학술지(정기간행물)] - Kanda N / 2004 / Histamine enhances the production of granulocytemacrophage colony-stimulating factor via protein kinase C alpha and extracellular signal-regulated kinase in human keratinocytes / J Invest Dermatol 122 : 863 ~ 872
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UCI(KEPA) : I410-ECN-0101-2018-524-001597904