지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 87 - 95 (9page)
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초록· 키워드
오류제보하기
It was previously observed that recombinant flock house virus (FHV) RNA1 was efficiently packaged into turnip yellow mosaic virus (TYMV), provided that the TYMV coat protein (CP) sequence was present at the 3"-end. FHV RNA encapsidated by TYMV CPs also had a four-nucleotide extension at the 5"-end. Since even a short extension at the 5"- and 3"-ends of FHV RNA1 inhibits replication, we examined whether the recombinant FHV RNA is indeed capable of replication. To this end, we introduced constructs expressing recombinant FHV RNAs into the plant Nicotiana benthamiana. Northern blot analysis of inoculated leaves suggested abundant production of recombinant FHV RNA1 and its subgenomic RNA. This demonstrated that recombinant FHV RNA with terminal extensions at both ends was competent for replication. We also showed that the recombinant FHV RNA can express the reporter gene encoding enhanced green fluorescent protein.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)] - Geall AJ / 2013 / RNA: The new revolu-tion in nucleic acid vaccines / Semin Immunol 25 : 152 ~ 159
[학술지(정기간행물)] - Sahin U / 2014 / mRNA-based therapeutics - developing a new class of drugs / Nat Rev Drug Discov 13 : 759 ~ 780
[학술지(정기간행물)] - Smith ML / 2007 / Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens / Virology 358 : 321 ~ 333
[학술지(정기간행물)] - Azizgolshani O / 2013 / Reconstituted plant viral capsids can release genes to mammalian cells / Virology 441 : 12 ~ 17
[학술지(정기간행물)] - Hyun-Il Shin / 2008 / Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA / BMB reports 41 (10) : 739 ~ 744
[학술지(정기간행물)] - Sahin U / 2014 / mRNA-based therapeutics - developing a new class of drugs / Nat Rev Drug Discov 13 : 759 ~ 780
[학술지(정기간행물)] - Smith ML / 2007 / Assembly of trans-encapsidated recombinant viral vectors engineered from Tobacco mosaic virus and Semliki Forest virus and their evaluation as immunogens / Virology 358 : 321 ~ 333
[학술지(정기간행물)] - Azizgolshani O / 2013 / Reconstituted plant viral capsids can release genes to mammalian cells / Virology 441 : 12 ~ 17
[학술지(정기간행물)] - Hyun-Il Shin / 2008 / Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA / BMB reports 41 (10) : 739 ~ 744
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UCI(KEPA) : I410-ECN-0101-2018-475-000980583