지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.12
- 수록면
- 137 - 146 (10page)
이용수
초록· 키워드
오류제보하기
Native turnip yellow mosaic virus (TYMV) is relatively unreactive to maleimide agents, indicating few reactive thiol groups on TYMV. In the present study, we aimed to construct TYMV mutants that have reactive cysteine residues on the surface. To this end, we prepared a library of TYMV mutants where the Thr residue at the C-terminus of coat protein (CP) was replaced by a random sequence of six amino acids that included one cysteine. This library was introduced into Nicotiana benthamiana by agroinfiltration. The CP sequence of the TYMV RNA isolated from inoculated leaves was amplified by reverse transcription-PCR and then used to construct a second library. This process was repeated one more time, and the CP sequences of the TYMV RNA in the inoculated leaves were analyzed. Based on the analysis of over 11,000 CP sequences, the Cys mutants representing most abundant TYMV RNAs were constructed. Analysis of the mutants showed that four Cys mutants were nearly comparable to wildtype with respect to CP and viral RNA levels in N. benthamiana. All these mutants were highly reactive to fluoresceine-5-maleimide. This demonstrates that TYMV can be modified to have additional functional groups on the surface that would be useful for drug delivery.
목차
INTRODUCTION
MATERIALS AND METHODS
DISCUSSION
REFERENCES
참고문헌 (20)
참고문헌 신청
[학술지(정기간행물)] - Dreher TW. / 2004 / Turnip yellow mosaic virus: transfer RNA mimicry, chloroplasts and a C-rich genome / Mol Plant Pathol 5 : 367 ~ 375
[학술지(정기간행물)] - Steinmetz NF / 2007 / Utilization of plant viruses in bionanotechnology / Org Biomol Chem 5 : 2891 ~ 2902
[학술지(정기간행물)] - Yildiz I / 2011 / Applications of viral nanoparticles in medicine / Curr Opin Biotechnol 22 : 901 ~ 908
[학술지(정기간행물)] - Wang Q / 2002 / Icosahedral virus particles as addressable nanoscale building blocks / Angew Chem Int Endl 41 : 459 ~ 462
[학술지(정기간행물)] - Steinmetz NF. / 2010 / Viral nanoparticles as platforms for next-generation therapeutics and imaging devices / Nanomedicine 6 : 634 ~ 641
[학술지(정기간행물)] - Steinmetz NF / 2007 / Utilization of plant viruses in bionanotechnology / Org Biomol Chem 5 : 2891 ~ 2902
[학술지(정기간행물)] - Yildiz I / 2011 / Applications of viral nanoparticles in medicine / Curr Opin Biotechnol 22 : 901 ~ 908
[학술지(정기간행물)] - Wang Q / 2002 / Icosahedral virus particles as addressable nanoscale building blocks / Angew Chem Int Endl 41 : 459 ~ 462
[학술지(정기간행물)] - Steinmetz NF. / 2010 / Viral nanoparticles as platforms for next-generation therapeutics and imaging devices / Nanomedicine 6 : 634 ~ 641
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UCI(KEPA) : I410-ECN-0101-2019-475-000334034