지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.2
- 수록면
- 85 - 93 (9page)
이용수
초록· 키워드
오류제보하기
Proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. For detection of antigens from Haemaphysalis longicornis, 1-dimensional electrophoresis (1-DE) quantitative immunoblotting technique combined with 2-dimensional electrophoresis (2-DE) immunoblotting was used for whole body proteins from unfed and partially fed female ticks. Reactivity bands and 2-DE immunoblotting were performed following 2-DE electrophoresis to identify protein spots. The proteome of the partially fed female had a larger number of lower molecular weight proteins than that of the unfed female tick. The total number of detected spots was 818 for unfed and 670 for partially fed female ticks. The 2-DE immunoblotting identified 10 antigenic spots from unfed females and 8 antigenic spots from partially fed females. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF) of relevant spots identified calreticulin, putative secreted WC salivary protein, and a conserved hypothetical protein from the National Center for Biotechnology Information and Swiss Prot protein sequence databases. These findings indicate that most of the whole body components of these ticks are non-immunogenic. The data reported here will provide guidance in the identification of antigenic proteins to prevent infestation and diseases transmitted by H. longicornis.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (35)
참고문헌 신청
Wang H / 2001 / Molecular individuality and adaptation of the tick Rhipicephalus appendiculatus in changed feeding environments / Med Vet Entomol 15:403~412
Xu G / 2004 / Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species / J Parasitol 90:73~78
Imamura S / 2009 / Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis / Exp Appl Acarol 48:345~358
You M / 2009 / Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari: Ixodidae) / J Vet Med Sci 71:709~712
You M / 2001 / Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis / Insect Biochem Mol Biol 32:67~73
Xu G / 2004 / Cloning and sequencing of putative calreticulin complementary DNAs from four hard tick species / J Parasitol 90:73~78
Imamura S / 2009 / Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis / Exp Appl Acarol 48:345~358
You M / 2009 / Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari: Ixodidae) / J Vet Med Sci 71:709~712
You M / 2001 / Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis / Insect Biochem Mol Biol 32:67~73
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UCI(KEPA) : I410-ECN-0101-2016-513-001133954