Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)

Tiantian Zhang; Xuejiao Cui; Jincheng Zhang; Hui Wang; Meng Wu; Hua Zeng; Yuanyuan Cao; Jingze Liu; Yonghong Hu

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자료유형: 학술저널

저자정보: Tiantian Zhang (Hebei Normal University) Xuejiao Cui (Hebei Normal University) Jincheng Zhang (Shijiazhuang Post and Telecommunication Technical College) Hui Wang (Hebei Normal University) Meng Wu (Hebei Academy of Sciences) Hua Zeng (Hebei Normal University) Yuanyuan Cao (Hebei Normal University) Jingze Liu (Hebei Normal University) Yonghong Hu (Hebei Normal University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.6

발행연도: 2015.12

수록면: 789 - 793 (5page)

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In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.

#Dermacentor silvarum #tick #antigenic protein #LC-MS/MS

참고문헌 (25)

참고문헌 신청

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