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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.10
- 수록면
- 337 - 344 (8page)
이용수
초록· 키워드
오류제보하기
In a previous study, we reported our discovery of Acanthamoeba contamination in domestic tap water; in that study, we determined that some Acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by mitochondrial DNA restriction fragment length polymorphism (Mt DNA RFLP). Five (29.4%) among 17 Acanthamoeba isolates contained endosymbionts in their cytoplasm, as demonstrated via orcein staining. In order to estimate their pathogenicity, we conducted a genetic characterization of the endosymbionts in Acanthamoeba isolated from domestic tap water via 16S rDNA sequencing. The endosymbionts of Acanthamoeba sp. KA/WP3 and KA/WP4 evidenced the highest level of similarity, at 97% of the recently published 16S rDNA sequence of the bacterium, Candidatus Amoebophilus asiaticus. The endosymbionts of Acanthamoeba sp. KA/WP8 and KA/WP12 shared a 97% sequence similarity with each other, and were also highly similar to Candidatus Odyssella thessalonicensis, a member of the a-proteobacteria. The endosymbiont of Acanthamoeba sp. KA/WP9 exhibits a high degree of similarity (85-95%) with genus Methylophilus, which is not yet known to harbor any endosymbionts. This is the first report, to the best of our knowledge, to show that Methylophilus spp. can live in the cytoplasm of Acanthamoeba.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (33)
참고문헌 신청
1. [학술지(정기간행물)] - Schuster FL / 2004 / Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals / Int J Parasitol 34 : 1001 ~ 1027
2. [학술지(정기간행물)] - Miltner EC / 2000 / Mycobacterium avium grown in Acanthamoeba castellanii is protected from the effects of antimicrobials / Antimicrob Agents Chemother 44 : 1990 ~ 1994
3. [학술지(정기간행물)] - Abd H / 2007 / Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii / FEMS Microbiol Ecol 60 : 33 ~ 39
4. [학술지(정기간행물)] - Ly TM / 1990 / Ingested Listeria monocytogenes survive and multiply in protozoa / J Med Microbiol 33 : 51 ~ 54
5. [학술지(정기간행물)] - Neumeister B / 2004 / Intracellular multiplication of Legionella species and the influence of amoebae on their intracellular growth in human monocytes: mono mac 6 cells and Acanthamoeba castellanii as suitable in vitro models / Methods Mol Biol 268 : 141 ~ 151
2. [학술지(정기간행물)] - Miltner EC / 2000 / Mycobacterium avium grown in Acanthamoeba castellanii is protected from the effects of antimicrobials / Antimicrob Agents Chemother 44 : 1990 ~ 1994
3. [학술지(정기간행물)] - Abd H / 2007 / Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii / FEMS Microbiol Ecol 60 : 33 ~ 39
4. [학술지(정기간행물)] - Ly TM / 1990 / Ingested Listeria monocytogenes survive and multiply in protozoa / J Med Microbiol 33 : 51 ~ 54
5. [학술지(정기간행물)] - Neumeister B / 2004 / Intracellular multiplication of Legionella species and the influence of amoebae on their intracellular growth in human monocytes: mono mac 6 cells and Acanthamoeba castellanii as suitable in vitro models / Methods Mol Biol 268 : 141 ~ 151
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UCI(KEPA) : I410-ECN-0101-2014-510-002532194