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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.6
- 수록면
- 269 - 277 (9page)
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Amoebic keratitis (AK) caused by Acanthamoeba is one of the most serious corneal infections. AK is frequently misdiagnosed initially as viral, bacterial, or fungal keratitis, thus ensuring treatment delays. Accordingly, the early detection of Acanthamoeba would contribute significantly to disease management and selection of an appropriate anti-amoebic therapy. Recently, the loop-mediated isothermal amplification (LAMP) method has been applied to the clinical diagnosis of a range of infectious diseases. Here, we describe a rapid and efficient LAMP-based method targeting Acanthamoeba 18S rDNA gene for the detection of Acanthamoeba using clinical ocular specimens in the diagnosis of AK. Acanthamoeba LAMP assays detected 11 different strains including all AK-associated species. The copy number detection limit for a positive signal was 10 DNA copies of 18S rDNA per reaction. No cross-reactivity with the DNA of fungi or other protozoa was observed. The sensitivity of LAMP assay was higher than those of Nelson primer PCR and JDP primer PCR. In the present study, LAMP assay based on directly heat-treated samples was found to be as efficient at detecting Acanthamoeba as DNA extracted using a commercial kit, whereas PCR was only effective when commercial kit-extracted DNA was used. This study showed that the devised Acanthamoeba LAMP assay could be used to diagnose AK in a simple, sensitive, and specific manner.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
REFERENCES
참고문헌 (28)
참고문헌 신청
1. [학술지(정기간행물)] - Khan NA / 2006 / Acanthamoeba: biology and increasing importance in human health / FEMS Microbiol Rev 30 : 564 ~ 595
2. [학술지(정기간행물)] - Corsaro D / 2010 / Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida) / Parasitol Res 107 : 233 ~ 238
3. [학술지(정기간행물)] - Nuprasert W / 2010 / Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand / J Clin Microbiol 48 : 4636 ~ 4640
4. [학술지(정기간행물)] - Ledee DR / 1996 / Acanthamoeba griffini. Molecular characterization of a new corneal pathogen / Invest Ophthalmol Vis Sci 37 : 544 ~ 550
5. [학술지(정기간행물)] - Walochnik J / 2000 / Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp / Appl Environ Microbiol 66 : 4408 ~ 4413
2. [학술지(정기간행물)] - Corsaro D / 2010 / Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida) / Parasitol Res 107 : 233 ~ 238
3. [학술지(정기간행물)] - Nuprasert W / 2010 / Identification of a novel T17 genotype of Acanthamoeba from environmental isolates and T10 genotype causing keratitis in Thailand / J Clin Microbiol 48 : 4636 ~ 4640
4. [학술지(정기간행물)] - Ledee DR / 1996 / Acanthamoeba griffini. Molecular characterization of a new corneal pathogen / Invest Ophthalmol Vis Sci 37 : 544 ~ 550
5. [학술지(정기간행물)] - Walochnik J / 2000 / Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp / Appl Environ Microbiol 66 : 4408 ~ 4413
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UCI(KEPA) : I410-ECN-0101-2014-510-002777699