지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.9
- 수록면
- 563 - 573 (11page)
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초록· 키워드
오류제보하기
Purpose : The purpose of this study is to investigate the effects of Simvastatin, which is HMG-CoA reductase inhibitor, on proliferation and differentiation of osteoblast.
Materials & Methods: Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 × 10⁴ cells per plate. Each plates were incubated with 5% CO² incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, 100μM of Simvastatin were added with Osteogenesis induction media, and classified as “test group”. Those not added with Simvastatin were classified as “control group”.
Results:
1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than 0.1μM.
2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than 0.1uM.
3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than 0.1μM.
4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group.
Conclusion: From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts.
Materials & Methods: Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 × 10⁴ cells per plate. Each plates were incubated with 5% CO² incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, 100μM of Simvastatin were added with Osteogenesis induction media, and classified as “test group”. Those not added with Simvastatin were classified as “control group”.
Results:
1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than 0.1μM.
2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than 0.1uM.
3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than 0.1μM.
4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group.
Conclusion: From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts.
목차
ABSTRACT
Ⅰ. 서론
Ⅱ. 연구재료 및 방법
Ⅲ. 연구 결과
Ⅳ. 총괄 및 고찰
Ⅴ. 결론
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UCI(KEPA) : I410-ECN-0101-2013-515-003365036