지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.10
- 수록면
- 623 - 632 (10page)
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초록· 키워드
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Purpose : The purpose of this study is to identify the effect of zoledronate(Zometa<SUP>®</SUP>), which is most common nitrogen containing bisphosphonate, on survival, proliferation, and differentiation of osteoblast.
Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 × 10⁴ cells per plates. Each plates were incubated with 5% CO² incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, 3 μM of zoledronate(Zometa<SUP>®</SUP>), every 2 days, for 12 days. “Control group” was plates not added with zoledronate(0μM), and “experiment group” were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, 3μM). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405㎚ with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast.
Results : Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased.
Conclusion : From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate.
Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 × 10⁴ cells per plates. Each plates were incubated with 5% CO² incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, 3 μM of zoledronate(Zometa<SUP>®</SUP>), every 2 days, for 12 days. “Control group” was plates not added with zoledronate(0μM), and “experiment group” were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, 3μM). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405㎚ with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast.
Results : Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased.
Conclusion : From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate.
목차
ABSTRACT
Ⅰ. 서론
Ⅱ. 연구재료 및 방법
Ⅲ. 연구 결과
Ⅳ. 총괄 및 고찰
Ⅴ. 결론
참고문헌
참고문헌 (49)
참고문헌 신청
1. [학술지(정기간행물)] - Menschutkin M / 1865 / Ueber die Einwirkung des Chloracetyls auf phophorige Saure / Ann Chem Pharm 133 : 317 ~ 320
2. [학술지(정기간행물)] - Fleisch H / 1968 / The influence of pyrophosphate analogues(diphosphates) on the precipitation and dissolution / Calcif Tissue Res : 10 ~ 10a
3. [학술지(정기간행물)] - Smith R / 1973 / Paget’s disease of bone, Experience with a disphosphonate(disodium etidronate) in treatment / Q J Med 42 : 235 ~ 256
4. [학술지(정기간행물)] - Russell RGG / 1974 / Disphosphonates in Paget’s disease / Lancet 1 (7863) : 894 ~ 898
5. [단행본] - Fleisch H / 2000 / Bisphosphonates in Bone Disease. From the Laboratory to the Patient. 4th / San Diego, CA: Academic Press
2. [학술지(정기간행물)] - Fleisch H / 1968 / The influence of pyrophosphate analogues(diphosphates) on the precipitation and dissolution / Calcif Tissue Res : 10 ~ 10a
3. [학술지(정기간행물)] - Smith R / 1973 / Paget’s disease of bone, Experience with a disphosphonate(disodium etidronate) in treatment / Q J Med 42 : 235 ~ 256
4. [학술지(정기간행물)] - Russell RGG / 1974 / Disphosphonates in Paget’s disease / Lancet 1 (7863) : 894 ~ 898
5. [단행본] - Fleisch H / 2000 / Bisphosphonates in Bone Disease. From the Laboratory to the Patient. 4th / San Diego, CA: Academic Press
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UCI(KEPA) : I410-ECN-0101-2013-515-003599925