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학술저널
저자정보
Ae-Jung Kim (혜전대학) Myung-Ryun Han (혜전대학) Myung-Hwan Kim (단국대학교) Myoungsook Lee (성신여자대학교) Taek-Joon Yoon (유한대학교) Sang-Do Ha (중앙대학교)
저널정보
한국영양학회 Nutrition Research and Practice Nutrition Research and Practice Vol.4 No.1
발행연도
2010.2
수록면
30 - 35 (6page)

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Our study focused on the antioxidant activities of Mosidae leaf ethanol extract (MLE) and included measurements of reducing power, total phenolic compounds, DPPH radical scavenging activity, and hydroxyl radical scavenging activity. In order to determine whether or not MLE evidences any chemopreventive activities, experimental lung metastasis was induced via the i.v. inoculation of colon26-M3.1 carcinoma cells into BALB/c mice. Additionally, we attempted to characterize any possible cytotoxic effects in murine normal splenocytes and tumor cells (B16-BL6 and colon26-M3.1). The total phenolic content and reducing capacity were measured at 39 ㎎/100 ㎖ and 1.24, respectively, whereas the DPPH and hydroxyl radical scavenging activities of MLE were measured to be 88.89% and 22.10%, respectively. Prophylactic i.v. treatment with MLE resulted in a dose-dependent and significant inhibition of lung metastasis. Specifically, a MLE dose of 200 ㎍ per mouse resulted in an 88.90% inhibition of lung metastasis. For the cytotoxicity assay, MLE doses up to 100 ㎍/㎖ were not shown to affect the growth of normal murine splenocytes. Additionally, the survival of normal cells was not affected at MLE doses below 500 ㎍/㎖. However, MLE doses up to 500 ㎍/㎖ reduced the percentage of tumor cell growth for B16BL6 (67% alive) and colon26-M3.1 (62% alive) cells.

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Abstract
Introduction
Materials and Methods
Results
Discussion
References

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UCI(KEPA) : I410-ECN-0101-2012-594-004464859