개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.9
- 수록면
- 171 - 175 (5page)
이용수
초록· 키워드
오류제보하기
The human genome contains approximately 13 orphan cytochrome P450 (P450, CYP) genes, of which the apparent function or substrate has not been identified. However, they seem to possess their own biological relevance in some tissues or developmental stages. Here, we characterized the heterologously expressed CYP2W1, an orphan P450 enzyme. The recombinant CYP2W1 protein containing a 6 × (His)-tag at Nterminus has been expressed in Escherichia coli and purified. Expression level of CYP2W1 holoenzyme was around 500 nmol P450 holoenzyme per liter culture medium. The reduced CO difference spectrum of CYP2W1 showed a maximum absorption at 449 ㎚. CYP2W1 indicated the significant induction to bioactivate Trp-P-1, MeIQ, and IQ in E. coli DJ701 tester strain. However, the bioactivation of B[α]P, and NNK by CYP2W1 was relatively low. The model structure of CYP2W1 suggested the characteristic P450 folds with the lengths and orientations of the individual secondary elements. The F-G loop is situated on the distal side of heme to accommodate the flexibility of active site of CYP2W1. These studies can provide useful information for the finding of its biological roles and structure-function relationships of an orphan CYP2W1 enzyme.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSIONS
ACKNOWLEDGMENTS
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
한약재의 Cytochrome P450 결합관련 안전성에 관한 연구
대한한방내과학회지
2013 .01
-
Effects of CYP1A2$^*$1C and CYP1A2$^*$1F Genotypes on the Activity and Inducibility of CYP1A2 Determined by Urinary Caffeine Metabolite Ratio in Koreans
Molecular & cellular toxicology
2007 .01
-
CYP2E15B, CYP2E16, CYP2E17B, CYP2E12, and CYP2E13 Allele Frequencies in Iranian Populations
Asian Pacific journal of cancer prevention : APJCP
2012 .01
-
Effects of Korean traditional herbal formula for common cold on the activities of human CYP450 isozymes
대한한의학회지
2014 .06
-
Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
Toxicological Research
2011 .03
-
CYP2E1과 CYP2C19 유전자 다형성이 위암 발생 감수성에 미치는 영향
Annals of Surgical Treatment and Research
2007 .08
-
한국인 정신분열병 환자의 지연성 운동장애와 $CYP2D6^*4$ 및 $CYP2D6^*10$ 다형성들의 연합에 대한 고찰
생물정신의학
2000 .01
-
약물대사효소 CYP2C19, CYP2D6의 다형성과 사상체질의 관련성 연구
대한한의학회지
2007 .03
-
Analysis of CYP17, CYP19 and CYP1A1 Gene Polymorphisms in Iranian Women with Breast Cancer
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Relationship between CYP17 and CYP11${\alpha}$, and PCOS in Korean Population
대한생식의학회 학술대회
2003 .01
-
암브록솔과 세티리진의 Cytochrome P450 저해 활성 평가
약학회지
2013 .01
-
Effects of Hydroxyl Group Numbers on the B-Ring of 5,7-Dihydroxyflavones on the Differential Inhibition of Human CYP 1A and CYP1B1 Enzymes
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2005 .01
-
Association of CYP2C19*2 and *3 Genetic Variants with Essential Hypertension in Koreans
Yonsei Medical Journal
2012 .01
-
Simultaneous determination of seven major human cytochrome P450 activities using LC/MS/MS
대한약학회 학술대회
2002 .01
-
CYP2C19 Polymorphism in Korean Patients on Warfarin Therapy
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2007 .01
-
Preferential Induction of CYP1A1 over CYP1B1 in Human Breast Cancer MCF-7 Cells after Exposure to Berberine
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
RNA Expression of Cytochrome P450 in Mexican Women with Breast Cancer
Asian Pacific journal of cancer prevention : APJCP
2012 .01
-
Roles of CYP2C9 and its variants (CYP2C9*2 and CYP2C9*3) in the metabolism of 6-methoxy-2-napthylacetic acid, an active metabolite of the prodrug nabumetone
Journal of Pharmaceutical Investigation
2020 .01
-
벤젠 대사에 있어서 CYP2E1 유전자다형성의 영향
Toxicological Research
2002 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2010-513-002817602