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자료유형
학술저널
저자정보
저널정보
한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY Vol.50 No.6
발행연도
2009.12
수록면
571 - 575 (5page)

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Transgenic cucumber (Cucumis sativus L.) plants were successfully obtained from cotyledon explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid pPTN161 containing nptII gene for resistance to kanamycin and BCL-2 gene related to apoptosis from human. To establish high frequency of transformation system forcucumber, various conditions for transformation were investigated. After co-cultivation for 48 hours, the cotyledon explants were placed on shoot induction media (MS, 3 ㎎ㆍL?¹ zeatin, 0.3 ㎎ㆍL?¹ IAA) containing 100 ㎎ㆍL?¹ kanamycin and 300 ㎎ㆍL?¹ lillacilin. Shoot induction was continued for 3-4 weeks, then subcultured once, and after 2 weeks the shoots were transferred to root induction medium. After culture and selection on MS medium supplemented with 3 ㎎ㆍL?¹ zeatin, 0.3 ㎎ㆍL?¹ IAA and 100 ㎎ㆍL?¹ kanamycin, a number of kanamycin-resistant plantlets were regenerated. Polymerase chain reaction, Southern blotting analyses were used to identify and characterize the transgenic plants with the integrated human BCL-2 gene. Over 10 transgenic plants have been established in soil and flowered in the greenhouse. This procedure facilitates introduction of another desirable gene into commercial inbred lines of cucumber.

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Abstract
Introduction
Materials and Methods
Results and Discussion
Ackknowledgement
Literature Cited

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