지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국원예학회 HORTICULTURE ENVIRONMENT and BIOTECHNOLOGY JOURNAL OF THE KOREAN SOCIETY FOR HORTICULTURAL SCIENCE Vol.46 No.3
- 발행연도
- 2005.6
- 수록면
- 169 - 175 (7page)
이용수
초록· 키워드
오류제보하기
The amorpha-4, 11-diene synthase gene isolated from Artemisia annua was inserted into the binary vector pILTAB 357 within restriction enzyme sites, EcoRI and BamHI. It was introduced into the lettuce through Agrobacterium-mediated transformation. A total of nine transgenic plants were obtained from the inoculated lettuce leaf disc. The integration of the amorpha-4, diene synthase gene into the lettuce genome was confirmed by polymerase chain reaction (PCR) and Southern hybridization. The PCR products of 1.6 kb have been produced from all transgenic plants. From the Southern blot analysis, the amorpha-4, 11-diene synthase gene was transferred to nine transgenic lettuce plants with one to three copies. The antibiotics resistance of T1 transgenic plants was tested by growing on medium supplemented with kanamycin sulfate (0, 50, and 100 ㎎ㆍL?¹). In the result, the segregation ratio of T1 transgenic plants was exhibited about 2.7:1, 638 individuals of kanamycin-resistant and 236 of kanamycin-sensitive. The inheritance characteristics of amorpha-4, 11-diene synthase gene in the T1 transgenic plants was analyzed by PCR. The 1.6 kb PCR products were produced from nine lines of T1 transgenic plants and its segregation ratio was 1:1. The artemisinin content of T1 transgenic lettuces was analyzed through gas chromatography and mass spectrometry. The gas chromatography analysis showed that the multi-peaks were produced from nine lines of T1 transgenic plants at a retention time of 10 min 7 s. Thus, mass spectrometric analysis of the T1 transgenic plants was performed. In the result, however, no T1 transgenic plants produce any peak corresponding to artemisinin.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Acknowledgements
Literature Cited
참고문헌 (17)
참고문헌 신청
[학술지(정기간행물)] - / 1992 / Qinghaosu for malaria : 649 ~ 651
[학술지(정기간행물)] - / 2001 / Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and artemisinin production in Artemisia annua 81 : 378 ~ 382
[학술지(정기간행물)] - / / Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases. 270 : 7375 ~ 7381
[학술지(정기간행물)] - / 1998 / Cloning and bacterial expression of sesquiterpene clyclase a key branch point enzyme for the synthesis of sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of Capsicum annuum 39 : 899 ~ 904
[학술지(정기간행물)] - / 1995 / Hairy root culture of Artemisina annua L. by Ri plasmid transformation and biosynthesis of artemisinin 11 : 227 ~ 235
[학술지(정기간행물)] - / 2001 / Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and artemisinin production in Artemisia annua 81 : 378 ~ 382
[학술지(정기간행물)] - / / Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases. 270 : 7375 ~ 7381
[학술지(정기간행물)] - / 1998 / Cloning and bacterial expression of sesquiterpene clyclase a key branch point enzyme for the synthesis of sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of Capsicum annuum 39 : 899 ~ 904
[학술지(정기간행물)] - / 1995 / Hairy root culture of Artemisina annua L. by Ri plasmid transformation and biosynthesis of artemisinin 11 : 227 ~ 235
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UCI(KEPA) : I410-ECN-0101-2009-525-016930515