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This study investigated the optimal conditions of high yield production of cyclofructan (CF) using recombinant deletion mutant enzyme CFT108 which is constructed by N-terminal deletion from cycloinulooligosaccharide fructanotransferase (CFTase) gene of Penibacillus polymyxa. The production yield was dependent on reaction time, substrate concentration and enzyme concentration. The optimum reaction time for industrial purpose was achieved at 3 hr reaction. The optimal concentrations of substrate and enzyme were found to be 2% inulin and 40 unit/g inulin, respectively. At optimum condition, 9.5 g/l of maximum yield and 47.5% of conversion efficacy were achieved. For purification of CF produced, the reaction mixture was treated with 1 unit/ml exoinulinase and then added 3% CaO three times with blowing CO₂gas, resulted in 95% purity.
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Abstract
재료 및 방법
결과 및 고찰
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감사의 글
참고문헌
참고문헌 (11)
참고문헌 신청
Jeon, S. J., D. J. You, H. J. Kwon, K. Shigenori, K. Namio, K. H. Kim, Y. H. Kim, and B. W. Kim. 2002. Cloning and characterization of Cycloinulooligosaccharide Fructa- notransferase(CFTase) from Bacillus polymyxsa MGL21. J. Microbiol. Biotechnol. 12(6),
Kanai, T., N. Ueki, T. Kawaguchi, Y. Teranishi, H. Atomi, C. Tomorbaatar, M. Ueda, and A. Tanaka. 1997. Recombinant thermostable cycloinulooligosaccharide fructanotransferase produced by Saccharomyces cerevisiae. Appl. Environ. Microbiol. 63, 4956-4960.
Kawamura, M., T. Uchiyama, T. Kuramoto, Y. Tamura, and K. Mizutani. 1989. Formation of a cycloinulo-oligosaccharide from inulin by an extracellular enzyme of Bacillus circulans OKUMZ31B. Carbohydr. Res. 192, 83-90.
Kawamura, M, and T. Uchiyama. 1994. Purification and some properties of cycloinulo- oligosaccharide fructanotransferase from Bacillus circulans OKUMZ31B. Carbohydr. Res. 260, 297-304.
Kim, H. Y, and Y. J. Choi. 2001. Molecular characterization of cycloinulooligosacc- haride fructanotransferase from Bacillus macerans. Appl. Environ. Microbiol. 67, 995-1000.
Kanai, T., N. Ueki, T. Kawaguchi, Y. Teranishi, H. Atomi, C. Tomorbaatar, M. Ueda, and A. Tanaka. 1997. Recombinant thermostable cycloinulooligosaccharide fructanotransferase produced by Saccharomyces cerevisiae. Appl. Environ. Microbiol. 63, 4956-4960.
Kawamura, M., T. Uchiyama, T. Kuramoto, Y. Tamura, and K. Mizutani. 1989. Formation of a cycloinulo-oligosaccharide from inulin by an extracellular enzyme of Bacillus circulans OKUMZ31B. Carbohydr. Res. 192, 83-90.
Kawamura, M, and T. Uchiyama. 1994. Purification and some properties of cycloinulo- oligosaccharide fructanotransferase from Bacillus circulans OKUMZ31B. Carbohydr. Res. 260, 297-304.
Kim, H. Y, and Y. J. Choi. 2001. Molecular characterization of cycloinulooligosacc- haride fructanotransferase from Bacillus macerans. Appl. Environ. Microbiol. 67, 995-1000.
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