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Isolation of Deletion Mutants by Reverse Genetics in Caenorhabditis elegans
Isolation of Deletion Mutants by Reverse Genetics in Caenorhabditis elegans
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2001.1
- 수록면
- 65 - 69 (5page)
이용수
Isolation of Deletion Mutants by Reverse Genetics in Caenorhabditis elegans
초록· 키워드
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Obtaining mutant animals is important for studying the function of a particular gene. A chemical mutagenesis was first carried out to generate mutations in C. elegans. In this study, we used ultraviolet-activated 4,5',8-trimethylpsoralen to induce small deletion mutations. A library of mutagenized worms was prepared for recovery of candidate animals and stored at $15^{\circ}C$ during screening instead of being made into a frozen stock library. In order to isolate deletion mutations in target genes, a polymerase chain reaction (PCR)-based screening method was used. As a result, two independent mutants with deletions of approximately 1.0 kb and 1.3 kb were isolated. This modified and improved reverse genetic approach was proven to be effective and practical for isolating mutant animals to study gene function at the organismal level.
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등록된 정보가 없습니다.
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