지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into an E. coli-yeast shuttle vector, pYES2.0, resulting in pYGECFTN. The plasmid pYGECFTN (8.6 kb) was introduced into Saccharomyces cerevisiae SEY2102 cells and then the transformants were selected on the synthetic defined media lacking uracil. The cft gene expression in yeast transformant was demonstrated by the analyses cyclofructan (CF) spots on thin-layer chromatogram. The recombinant CFTase was not secreted into the medium and localized in the periplasmic space. The production of CF was observed after 5 min of the enzymatic reaction with inulin. The optimun pH and temperature for CF production were found to be at pH 8.0 and 45℃, respectively. Enzyme activity was stably maintained up to 55℃. The CF was produced from all inulin sources and was most efficiently produced from dahlia tubers and Jerusalem artichokes.
목차
Abstract
재료 및 방법
결과 및 고찰
요약
감사의 글
참고문헌
참고문헌 (24)
참고문헌 신청
Bajpai, P. K. and P. Bajpai. 1991. Cultivation and utilization of Jerusalem artichoke for ethanol, single cell protein, and high fructose syrup production. Enz. Microbial. Technol. 13, 359-362.
Emr, S. D., R. Schekman, M. C. Flessel and J. Thorner. 1983. An MF α1-SUC2 ( α-factor invertase) gene fusion for study of protein localization and gene expression in yeast. Proc. Natl. Acad. Sci. USA. 80, 7080-7084.
Eom, S. J., Y. M. Kwon and Y. J. Choi. 1995. Molecular cloning of Pseudomonas sp. inulinase gene and its expression in E. coli. Kor J. Appl. Microbiol. Biotechnol. 23, 550-555.
Han, Y. J., D. O. Kang, S. C. Lee, B. Y. Kim, H. H. Suh, J. M. Kim, T. I. Mheen and J. S. Ahn. 1994. Secretion of a Bacillus endoglucanase in Saccharomyces cerevisiae by its own signal sequence. J. Microbiol. Biotechnol. 4, 24-29.
Hirst, E. L., D. J. Mcgilvary and E. G. V. Percival. 1950. Studies on fructosans. J. Chem. Soc. 72, 1279-1284.
Emr, S. D., R. Schekman, M. C. Flessel and J. Thorner. 1983. An MF α1-SUC2 ( α-factor invertase) gene fusion for study of protein localization and gene expression in yeast. Proc. Natl. Acad. Sci. USA. 80, 7080-7084.
Eom, S. J., Y. M. Kwon and Y. J. Choi. 1995. Molecular cloning of Pseudomonas sp. inulinase gene and its expression in E. coli. Kor J. Appl. Microbiol. Biotechnol. 23, 550-555.
Han, Y. J., D. O. Kang, S. C. Lee, B. Y. Kim, H. H. Suh, J. M. Kim, T. I. Mheen and J. S. Ahn. 1994. Secretion of a Bacillus endoglucanase in Saccharomyces cerevisiae by its own signal sequence. J. Microbiol. Biotechnol. 4, 24-29.
Hirst, E. L., D. J. Mcgilvary and E. G. V. Percival. 1950. Studies on fructosans. J. Chem. Soc. 72, 1279-1284.
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