목차

CHAPTER Ⅰ 1
GENERAL INTRODUCTION 2
1.1. Apple cultivars in Korea 2
1.2. 1-Methycyclopropene (1-MCP) application 4
1.3. Ethylene 5
1.4. Research objectives 5
1.5. References 6
CHAPTER Ⅱ 9
Differences in internal ethylene concentration and fruit quality attributes during cold storage in apple cultivars 10
2.1. Abstract 10
2.2. Introduction 11
2.3. Materials and methods 13
2.3.1. Plant materials and treatment 13
2.3.2. Assessment of fruit quality attributes 14
2.3.3. Statistical analysis 15
2.4. Results and discussion 15
2.5. References 29
CHAPTER Ⅲ 33
Effects of 1-methylcyclopropene treatment on fruit quality attributes during cold storage in apple cultivars 34
3.1. Abstract 34
3.2. Introduction 35
3.3. Materials and methods 36
3.3.1. Plant materials and treatment 36
3.3.2. Assessment of fruit quality attributes 36
3.3.3. Statistical analysis 38
3.4. Results and discussion 38
3.5. References 52
CHAPTER Ⅳ 55
Effects of 1-methylcyclopropene treatment on cell wall components and hydrolase activities during cold storage in apple cultivars 56
4.1. Abstract 56
4.2. Introduction 57
4.3. Materials and methods 58
4.3.1. Plant materials and treatment 58
4.3.2. Extraction of cell wall components 58
4.3.3. Determination of total sugar content 59
4.3.4. Determination of uronic acid 59
4.3.5. Extraction of cell wall hydrolases 60
4.3.6. Activities of cell wall hydrolases 60
4.3.7. Statistical analysis 61
4.4. Results and discussion 61
4.5. References 81
CHAPTER Ⅴ 85
Effects of 1-methylcyclopropene treatment on antioxidant metabolism during cold storage in apple cultivars 86
5.1. Abstract 86
5.2. Introduction 87
5.3. Materials and methods 88
5.3.1. Plant materials and treatment 88
5.3.2. Extraction of antioxidant metabolite 88
5.3.3. Determination of total phenolic compounds content 89
5.3.4. Determination of DPPH and ABTS radical scavenging activities 90
5.3.5. Statistical analysis 91
5.4. Results and discussion 91
5.5. References 108
CHAPTER Ⅵ 112
Effects of 1-methylcyclopropene treatment on ethylene response genes during cold storage in apple cultivars 113
6.1. Abstract 113
6.2. Introduction 114
6.3. Materials and methods 116
6.3.1. Plant materials and treatment 116
6.3.2. Gene expression analysis 117
6.3.3. Statistical analysis 118
6.4. Results and Discussion 118
6.5. References 131
CHAPTER Ⅶ 135
Statistical correlation on biochemical and genetic alterations of
fruit quality by 1-methylcyclopropene treatment during cold
storage in apple cultivars 136
7.1. Abstract 136
7.2. Statistical analysis 137
7.3. Results and Discussion 138
7.4. References 162
SUMMARY 165
국문 초록 168