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논문 기본 정보

자료유형
학술저널
저자정보
Kang Jungmok (Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea) Choi Daeho (Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea) Jung Eun-Suk (Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea) Park Yong-Woo (Researcher, Forest Bio Center, Chungcheongbuk-do Forest Environment Research Center, Okcheon 29061, Korea)
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제51권 제3호
발행연도
2024.9
수록면
228 - 236 (9page)
DOI
10.5010/JPB.2024.51.022.228

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초록· 키워드

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In this study, suitable culture media, growth regulators, and acclimatization soil for regenerated plants were evaluated to enable mass production of Rhododendron micranthum, and the contents of functional compounds at culture stage were analyzed to confirm industrial applicability. For shoot proliferation, culturing in 4 mg/L zeatin-containing Murashige and Skoog medium was found to be the most effective treatment; the shoot induction rate was 77.08%, number of developing shoots was 2.8, and length of shoots was 9.01 mm. Regarding culture medium and growth regulator suitable for rooting, half-strength Economou and Read medium and 1 mg/L indole-3-butyric acid treatment were the most effective, with a rooting rate of 95.83%, root number of 4.1, and root length of 12.59 mm. Peatmoss was found to be most effective for facilitating ex vitro accli- matization of regenerated plants, with a seedling shoot length of 69.27 mm and root length of 49.30 mm. Chlorogenic acid, astragalin, myricetin, and afzelin contents were analyzed via high-performance liquid chromatography analysis at culture stage. The in vitro-rooted seedlings contained 3.77 mg/g chlorogenic acid, 1.53 mg/g astragalin, 1.07 mg/g myricetin, and 0.17 mg/g afzelin; the contents were higher than those in in vitro shoots (0.44 mg/g chlorogenic acid, 1.30 mg/g astragalin, 1.04 mg/g myricetin, and 0.05 mg/g afzelin) and increased as the stage progressed. We confirmed that these culture conditions can be used for additional research, such as increasing the contents of functional compounds and mass production research. Based on these findings, there is a possibility of supplying raw materials and producting four functional compound using in vitro culture of R. micranthum.

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