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학술저널
저자정보
배기화 (국립세종수목원) 윤이슬 (국립세종수목원) Ji-Sun Jung (Department of Temperate Middle Part Plant Conservation Sejong National Arboretum Republic of Korea) Chan-Beom Kim (Department of Temperate Middle Part Plant Conservation Sejong National Arboretum Republic of Korea) Hye-Won Kim (Department of Temperate Middle Part Plant Conservation Sejong National Arboretum Republic of Korea) 홍용식 (국립세종수목원) Min-Kyeong Oak (Department of Temperate Middle Part Plant Conservation Sejong National Arboretum Republic of Korea) 김학구 (국립세종수목원) Ju-Hui Lee (Department of Temperate Middle Part Plant Conservation Sejong National Arboretum Republic of Korea)
저널정보
강원대학교 산림과학연구소 Journal of forest and environmental science Journal of forest and environmental science 제37권 제4호
발행연도
2021.12
수록면
304 - 308 (5page)

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Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

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