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논문 기본 정보

자료유형
학술저널
저자정보
Mosoh Dexter Achu (Centre for Biodiversity Exploration and Conservation (CBEC), 15, Kundan Residency, 4th Mile Mandla Road, Tilhari, Jabalpur, M.P, 482021, IndiaSchool of Sciences, Sanjeev Agrawal Global Educa) Khandel Ashok Kumar (Bhoomi Institute of Research in Advance Biotechnology (BIRAB), Plot No. Z-20, SF-5, A-2, 14 Badri Mahal, M.P. Nagar, Zone - I, Bhopal, M.P, 462011, India) Verma Sandeep Kumar (Institute of Biological Science, Sanjeev Agrawal Global Educational (SAGE) University, Indore, M.P, 452020, India) Vendrame Wagner A. (Environmental Horticulture Department, University of Florida, Institute of Food and Agricultural Sciences, 2550 Hull Rd., Gainesville, FL 32611, USA)
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제51권 제3호
발행연도
2024.9
수록면
237 - 252 (16page)
DOI
10.5010/JPB.2024.51.023.237

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Gloriosa superba L. is classified as an endangered species owing to slow natural propagation and widespread exploitation in the wild. Therefore, we aim to develop an efficient protocol for the in vitro regeneration of G. superba L. using leaf explants. Optimal callus induction was achieved using a combination of 1-naphthalene acetic acid (NAA) and kinetin (KN) [1.5 mg L NAA + 0.5 mg L KN was -1 -1 supplemented with 10 mg L casein hydrolysate (CH)]. This -1 formulation resulted in the swiftest initiation of callus formation (within 12 d) and yielded the highest callus induction rate (71.88%). Furthermore, addition of 5 mg L-1 CH and 20% (v/v) coconut water to Murashige and Skoog (MS) medium supplemented with 2.0 mg L 6-benzyl- aminopurine and 0.5 mg L NAA facilitated the formation of shoot primordia within 14 d, achieving the highest average number of shoots per callus (5.25). For root development, use of half-strength MS medium supple- mented with 1.0 mg L indole-3-butyric acid resulted in the -1 -1 -1 highest root-to-shoot ratio (5.75), root fresh weight (143 mg), and root dry weight (22.2 mg). The in vitro-cultivated plantlets had a 100% survival rate within three weeks of placement in culture rooms and shade net enclosures. After transplantation into a substrate comprising garden soil, sand, and vermiculite and exposure to direct sunlight, the plantlets achieved a 76% survival rate by the fifth week, thereby maintaining their typical growth characteristics. Our protocol enables large-scale production of genetically uniform G. superba L. plants. This demonstrates the potential of tissue culture techniques in plant propagation and biotechnological applications, thereby contributing to current understanding and paving the way for future research.

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