지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.1
- 수록면
- 1 - 5 (5page)
- DOI
- 10.1016/j.mocell.2024.100141
이용수
초록· 키워드
오류제보하기
RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature, sensitivity, and specificity. Here, we provide an overview of RT-qPCR, focusing on essential reagents, the importance of primer design, the detailed workflow, and data analysis methods. This guide will be useful for scientists who are unfamiliar with RT-qPCR, highlighting key considerations for successful RNA quantification.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
Journal of Microbiology and Biotechnology
2021 .01
-
Detection of Rift Valley Fever Virus Using RT-qPCR and RT-ddPCR
한국미생물학회 학술대회논문집
2022 .10
-
Evaluation of Various Real-Time Reverse Transcription Quantitative PCR Assays for Norovirus Detection
Journal of Microbiology and Biotechnology
2017 .01
-
Stem-loop RT-qPCR 분석법을 이용한 siRNA 치료제의 생체시료 분석법 검증 및 약물 동태학적 분석
생명과학회지
2019 .06
-
Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification
The Plant Pathology Journal
2015 .09
-
Effect of methemoglobin on carboxyhemoglobin (CO-Hb, %) quantification
한국분석과학회 학술대회
2023 .11
-
Functional genomics by integrated analysis of transcriptome of sweet potato (Ipomoea batatas (L.) Lam.) during root formation
Genes & Genomics
2020 .01
-
qPCR Based Quantification Method Validation for GM Canola DP073496-4 and MON88302
한국분석과학회 학술대회
2017 .11
-
Development and Application of Reverse Transcription Nanoplate-Based Digital PCR Assay for Sensitive and Accurate Detection of Rice Black-Streaked Dwarf Virus in Cereal Crops
The Plant Pathology Journal
2024 .08
-
Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량
Research in Plant Disease(식물병연구)
2021 .09
-
Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design
Genes & Genomics
2022 .08
-
Development of a One-Step Multiplex Reverse Transcription- Quantitative Polymerase Chain Reaction Assay for the Simultaneous Detection of Four Lily Viruses
Research in Plant Disease(식물병연구)
2024 .12
-
A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus
The Plant Pathology Journal
2023 .08
-
Quantitative Polymerase Chain Reaction for Microbial Growth Kinetics of Mixed Culture System
Journal of Microbiology and Biotechnology
2015 .01
-
Alternative Carcinogenicity Screening Assay Using Colon Cancer Stem Cells: A Quantitative PCR (qPCR)-Based Prediction System for Colon Carcinogenesis
Journal of Microbiology and Biotechnology
2018 .01
-
Visualization and Quantification of Non-coding Cellular RNA in a Single Cell Using Atomic Force Microscopy
한국분석과학회 학술대회
2019 .05
-
RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus
The Plant Pathology Journal
2024 .08
-
Analysis of Whole Transcriptome Sequencing Data: Workflow and Software
Genomics & informatics
2015 .01
-
Combining the Power of Advanced Proteome-wide Sample Preparation Methods and Mass Spectrometry for defining the RNA-Protein Interactions
Mass Spectrometry Letters
2022 .12
최근 본 자료
댓글(0)
0