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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제4호
- 발행연도
- 2017.1
- 수록면
- 816 - 824 (9page)
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초록· 키워드
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Human noroviruses are widespread and contagious viruses causing nonbacterial gastroenteritis. Real-time reverse transcription quantitative PCR (real-time RT-qPCR) is currently the gold standard for the sensitive and accurate detection of these pathogens and serves as a critical tool in outbreak prevention and control. Different surveillance teams, however, may use different assays, and variability in specimen conditions may lead to disagreement in results. Furthermore, the norovirus genome is highly variable and continuously evolving. These issues necessitate the re-examination of the real-time RT-qPCR’s robustness in the context of accurate detection as well as the investigation of practical strategies to enhance assay performance. Four widely referenced real-time RT-qPCR assays (Assays A-D) were simultaneously performed to evaluate characteristics such as PCR efficiency, detection limit, and sensitivity and specificity with RT-PCR, and to assess the most accurate method for detecting norovirus genogroups I and II. Overall, Assay D was evaluated to be the most precise and accurate assay in this study. A ZEN internal quencher, which decreases nonspecific fluorescence during the PCR, was added to Assay D’s probe, which further improved the assay performance. This study compared several detection assays for noroviruses, and an improvement strategy based on such comparisons provided useful characterizations of a highly optimized real-time RT-qPCR assay for norovirus detection.
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참고문헌 (24)
참고문헌 신청
Ahmed SM / 2014 / Global prevalence of norovirus in cases of gastroenteritis: a systematic review and metaanalysis / Lancet Infect. Dis 14 : 725 ~ 730
Atmar RL / 2008 / Norwalk virus shedding after experimental human infection / Emerg. Infect. Dis 14 : 1553 ~ 1557
Bustin SA / 2009 / The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments / Clin. Chem 55 : 611 ~ 622
Cheesbrough JS / 2000 / Widespread environmental contamination with Norwalk-like viruses (NLV) detected in a prolonged hotel outbreak of gastroenteritis / Epidemiol. Infect 1
Green K / 2013 / Fields Virology / Lippincott Williams & Wilkins : 583 ~ 609
Atmar RL / 2008 / Norwalk virus shedding after experimental human infection / Emerg. Infect. Dis 14 : 1553 ~ 1557
Bustin SA / 2009 / The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments / Clin. Chem 55 : 611 ~ 622
Cheesbrough JS / 2000 / Widespread environmental contamination with Norwalk-like viruses (NLV) detected in a prolonged hotel outbreak of gastroenteritis / Epidemiol. Infect 1
Green K / 2013 / Fields Virology / Lippincott Williams & Wilkins : 583 ~ 609
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