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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,928 - 1,935 (8page)
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Microbial growth kinetics is often used to optimize environmental processes owing to its relation to the breakdown of substrate (contaminants). However, the quantification of bacterial populations in the environment is difficult owing to the challenges of monitoring a specific bacterial population within a diverse microbial community. Conventional methods are unable to detect and quantify the growth of individual strains separately in the mixed culture reactor. This work describes a novel quantitative PCR (qPCR)-based genomic approach to quantify each species in mixed culture and interpret its growth kinetics in the mixed system. Batch experiments were performed for both single and dual cultures of Pseudomonas putida and Escherichia coli K12 to obtain Monod kinetic parameters (μmax and Ks). The growth curves and kinetics obtained by conventional methods (i.e., dry weight measurement and absorbance reading) were compared with that obtained by qPCR assay. We anticipate that the adoption of this qPCR-based genomic assay can contribute significantly to traditional microbial kinetics, modeling practice, and the operation of bioreactors, where handling of complex mixed cultures is required.
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참고문헌 (24)
참고문헌 신청
Baxter J / 2008 / The degradation of the herbicide bromoxynil and its impact of bacterial diversity in a top soil / J. Appl. Microbiol 104 : 1605 ~ 1616
Bekins BA / 1998 / A comparison of zero order, first order, and monod biotransforamtion models / Ground Water 36 : 261 ~ 268
Brown AM / 2001 / A step-by-step guide to non-linear regression analysis of experimental data using Microsoft Excel spreadsheet / Comput. Methods Programs Biomed 65 : 191 ~ 2
Dykhuizen D / 1978 / Selection for tryptophan auxotrophs of Escherichia coli in glucose-limited chemostats as a test of the energy conservation hypothesis of evolution / Evol
Egli T. / 2010 / How to live at very low substrate concentration / Water Res 44 : 4826 ~ 4837
Bekins BA / 1998 / A comparison of zero order, first order, and monod biotransforamtion models / Ground Water 36 : 261 ~ 268
Brown AM / 2001 / A step-by-step guide to non-linear regression analysis of experimental data using Microsoft Excel spreadsheet / Comput. Methods Programs Biomed 65 : 191 ~ 2
Dykhuizen D / 1978 / Selection for tryptophan auxotrophs of Escherichia coli in glucose-limited chemostats as a test of the energy conservation hypothesis of evolution / Evol
Egli T. / 2010 / How to live at very low substrate concentration / Water Res 44 : 4826 ~ 4837
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