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논문 기본 정보

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학술저널
저자정보
Kim Jeong-Min (Division of Emerging Infectious Diseases, Department of Disease Diagnosis and Analysis, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea) Kim Dongju (Division of Emerging Infectious Diseases, Department of Disease Diagnosis and Analysis, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea) Rhee Jee Eun (Division of Emerging Infectious Diseases, Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency (KDCA), Cheongju, Korea) Yoo Cheon Kwon (Department of Disease Diagnosis and Analysis, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea) Kim Eun-Jin (Division of Emerging Infectious Diseases, Department of Disease Diagnosis and Analysis, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea)
저널정보
질병관리본부 Osong Public Health and Research Persptectives Osong Public Health and Research Perspectives Vol.15 No.3
발행연도
2024.6
수록면
260 - 264 (5page)
DOI
10.24171/j.phrp.2023.0216

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Objectives: We analyzed the correlation between the infectivity and transmissibility of the severe acute respiratory syndrome coronavirus 2 Omicron sublineages BA.1, BA. 2, BA.4, and BA.5.Methods: We assessed viral replication kinetics and infectivity at the cellular level. Nasopharyngeal and oropharyngeal specimens were obtained from patients with coronavirus disease 2019, confirmed using whole-genome sequencing to be caused by the Omicron sublineages BA.1, BA.2, BA.4, or BA.5. These specimens were used to infect Vero E6 cells, derived from monkey kidneys, for the purpose of viral isolation. Viral stocks were then passaged in Vero E6 cells at a multiplicity of infection of 0.01, and culture supernatants were harvested at 12-hour intervals for 72 hours. To evaluate viral replication kinetics, we determined the cycle threshold values of the supernatants using real-time reverse transcription polymerase chain reaction and converted these values to genome copy numbers.Results: The viral load was comparable between BA.2, BA.4, and BA.5, whereas BA.1 exhibited a lower value. The peak infectious load of BA.4 was approximately 3 times lower than that of BA.2 and BA.5, while the peak load of BA.2 and BA.5 was about 7 times higher than that of BA.1. Notably, BA.1 demonstrated the lowest infectivity over the entire study period.Conclusion: Our results suggest that the global BA.5 wave may have been amplified by the higher viral replication and infectivity of BA.5 compared to other Omicron sublineages. These analyses could support the rapid assessment of the impact of novel variants on case incidence.

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