Chlorophyll contents and expression profiles of photosynthesis-related genes in water-stressed banana plantlets

Widiyanto Sri Nanan; Sulaiman Syahril; Duve Simon; Marwani Erly; Nugrahapraja Husna; Diningrat Diky Setya

doi:10.5010/JPB.2023.50.016.127

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자료유형: 학술저널

저자정보: Widiyanto Sri Nanan (School of Life Sciences and Technology Institut Teknologi Bandung Bandung 40132 Indonesia) Sulaiman Syahril (School of Life Sciences and Technology Institut Teknologi Bandung Bandung 40132 Indonesia) Duve Simon (School of Life Sciences and Technology Institut Teknologi Bandung Bandung 40132 Indonesia) Marwani Erly (School of Life Sciences and Technology Institut Teknologi Bandung Bandung 40132 Indonesia) Nugrahapraja Husna (School of Life Sciences and Technology Institut Teknologi Bandung Bandung 40132 Indonesia) Diningrat Diky Setya (Department of Biology Faculty of Mathematics and Natural Sciences Medan State University Medan 20221 Indonesia)

저널정보: 한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제50권 제2호

발행연도: 2023.6

수록면: 127 - 136 (10page)

DOI: 10.5010/JPB.2023.50.016.127

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Water scarcity decreases the rate of photo- synthesis and, consequently, the yield of banana plants (Musa spp). In this study, transcriptome analysis was performed to identify photosynthesis-related genes in banana plants and determine their expression profiles under water stress conditions. Banana plantlets were in vitro cultured on Murashige and Skoog agar medium with and without 10% polyethylene glycol and marked as BP10 and BK. Chlorophyll contents in the plant shoots were determined spectrophotometrically. Two cDNA libraries generated from BK and BP10 plantlets, respectively, were used as the reference for transcriptome data. Gene ontology (GO) enrichment analysis was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) and visualized using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway prediction. Morphological obser- vations indicated that water deficiency caused chlorosis and reduced the shoot chlorophyll content of banana plantlets. GO enrichment identified 52 photosynthesis-related genes that were affected by water stress. KEGG visualization revealed the pathways related to the 52 photosynthesis- related genes and their allocations in four GO terms. Four, 12, 15, and 21 genes were related to chlorophyll biosynthesis, the Calvin cycle, the photosynthetic electron transfer chain, and the light-harvesting complex, respectively. Differentially expressed gene (DEG) analysis using DESeq revealed that 45 genes were down-regulated, whereas seven genes were up-regulated. Four of the down-regulated genes were responsible for chlorophyll biosynthesis and appeared to cause the decrease in the banana leaf chlorophyll content. Among the annotated DEGs, MaPNDO, MaPSAL, and MaFEDA were selected and validated using quantitative real-time PCR.

#Musa acuminata Colla # Photosynthesis #Transcriptome analysis #Water deficiency

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