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논문 기본 정보

자료유형
학술저널
저자정보
Se Hyuk Oh (충북대학교) Sang Hun Park (충북대학교) 박윤환 (충북대학교) Yun A Kim (충북대학교) Gyu Tae Park (충북대학교) Xiang-Shun Cui (Chungbuk National University) Xiang-Shun Cui (Chungbuk National University) Kwan Suk Kim (Chungbuk National University) Seon-Tea Joo (Gyeongsang National University Korea) 허선진 (중앙대학교) 김갑돈 (서울대학교) 김갑돈 (서울대학교) 최정석 (충북대학교)
저널정보
한국축산학회(구 한국동물자원과학회) 한국축산학회지 한국축산학회지 제65권 제3호
발행연도
2023.5
수록면
664 - 678 (15page)

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To improve culture efficiency of Hanwoo myosatellite cells, these cells were cultured at different temperatures. Hanwoo myosatellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37℃ and 39℃ and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37℃ proliferated better than cultured at 39℃ (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in myotube thickness and Fusion index (p > 0.05). In Western blotting analysis, Hanwoo myosatellite cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatures (p > 0.05). In reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis, Hanwoo myosatellite cells cultured at 39℃ had significantly (p < 0.05) higher expression levels of MyHC, MYF6, and MB than those cultured at 37℃. C2C12 cells cultured at 39℃ showed significantly (p < 0.05) higher expression levels of MYOG and MB than those cultured at 37℃. To increase culture efficiency of Hanwoo myosatellite cells, proliferating at 37℃ and differentiating at 39℃ are appropriate. Since results of temperature differences of Hanwoo myosatellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.

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