지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제3호
- 발행연도
- 2023.3
- 수록면
- 378 - 386 (9page)
- DOI
- 10.4014/jmb.2301.01014
이용수
초록· 키워드
오류제보하기
The CYP707A family genes encoding ABA 8’-hydroxylase catabolize abscisic acid (ABA), a plant stress hormone that plays an important role in stress condition, such as drought, heat, cold and salinity. Phaseic acid (PA) is a catabolic product of ABA. Recent studies have shown that PA is important for the physiological functions in plants. It is also a neuroprotective molecule that protects against ischemic brain injury in mice. To obtain enzymes for the PA production, four CaCYP707A genes (CaCYP707A1, CaCYP707A2, CaCYP707A3 and CaCYP707A4) were isolated from hot pepper. They were heterologously expressed in Escherichia coli. Among them, CaCYP707A2 showed significantly higher expression levels in both the membrane fraction and the soluble fraction.
Preferred redox partners were investigated to improve the efficiency of CaCYP707A2's catalytic reaction, and NADPH-cytochrome P450 reductase (CPR) from hot pepper (CaCPR) was preferred over other redox partners (i.e., rat CPR and ferredoxin reductase/ferredoxin). The production of 8’- hydroxy ABA and PA by ABA hydroxylation activity was confirmed in CaCYP707A2 from both membrane and soluble fractions. Therefore, CaCYP707A2 is the first identified plant CYP protein that is expressed a soluble form in cytosolic fraction having stable activity. Taken together, we propose a new CYP707A protein with industrial applications for PA production without additional modifications in E. coli heterologous expression.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling
Molecules and Cells
2016 .02
-
Modification of N-Terminal Amino Acids of Fungal Benzoate Hydroxylase (CYP53A15) for the Production of p-Hydroxybenzoate and Optimization of Bioproduction Conditions in Escherichia coli
Journal of Microbiology and Biotechnology
2018 .01
-
Characterization of Two Self-Sufficient Monooxygenases, CYP102A15 and CYP102A170, as Long-Chain Fatty Acid Hydroxylases
Journal of Microbiology and Biotechnology
2020 .01
-
The phytohormone abscisic acid increases triacylglycerol content in the green microalga Chlorella saccharophila (Chlorophyta)
Algae
2016 .01
-
Domain Characterization of Cyclosporin Regio-Specific Hydroxylases in Rare Actinomycetes
Journal of Microbiology and Biotechnology
2015 .01
-
The Arabidopsis Phytocystatin AtCYS5 Enhances Seed Germination and Seedling Growth under Heat Stress Conditions
Molecules and Cells
2017 .08
-
Characterization of Gel16 as a Cytochrome P450 in Geldanamycin Biosynthesis and in-silico Analysis for an Endogenous Electron Transport System
Journal of Microbiology and Biotechnology
2019 .01
-
Evaluation of the inhibitory effect of Gynostemma pentaphyllum extracts on CYP450 enzyme activities using LC-MS/MS
Mass Spectrometry Letters
2023 .09
-
Arabidopsis MAP3K16 and Other Salt-Inducible MAP3Ks Regulate ABA Response Redundantly
Molecules and Cells
2017 .03
-
Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives
Journal of Microbiology and Biotechnology
2015 .01
-
식물의 물부족 스트레스 신호 전달 네트워크에 대한 이해
생명과학회지
2018 .03
-
Soluble Expression of Artificially Designed Recombinant Protein through Conjugating Fusion Protein in Escherichia coli
한국미생물학회 학술대회논문집
2017 .04
-
Arabidopsis Raf-Like Kinase Raf10 Is a Regulatory Component of Core ABA Signaling
Molecules and Cells
2019 .01
-
Crystal Structure and Biochemical Analysis of a Cytochrome P450 Steroid Hydroxylase ( Ba CYP106A6) from Bacillus Species
Journal of Microbiology and Biotechnology
2023 .03
-
Characterization of CYP125A13, the First Steroid C-27 Monooxygenase from Streptomyces peucetius ATCC27952
Journal of Microbiology and Biotechnology
2020 .01
-
Improved NADPH Regeneration for Fungal Cytochrome P450 Monooxygenase by Co-Expressing Bacterial Glucose Dehydrogenase in Resting-Cell Biotransformation of Recombinant Yeast
Journal of Microbiology and Biotechnology
2016 .12
-
Induced Tolerance to Salinity Stress by Halotolerant Bacteria Bacillus aryabhattai H19-1 and B. mesonae H20-5 in Tomato Plants
Journal of Microbiology and Biotechnology
2019 .01
-
Heterologous Expression of Rhizopus Oryzae CYP509C12 Gene in Rhizopus Nigricans Enhances Reactive Oxygen Species Production and 11α-Hydroxylation Rate of 16α, 17-Epoxyprogesterone
Mycobiology
2019 .01
-
H₂O₂-Driven Hydroxylation of Steroids Catalyzed by Cytochrome P450 CYP105D18: Exploration of the Substrate Access Channel
극지연구소 초록집
2024 .07
-
Molecular Cloning and Characterization of Bovine CYP26A1 Promoter
생명과학회지
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0