지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제1호
- 발행연도
- 2019.1
- 수록면
- 44 - 54 (11page)
이용수
초록· 키워드
오류제보하기
Geldanamycin and its derivatives, inhibitors of heat shock protein 90, are considered potent anticancer drugs, although their biosynthetic pathways have not yet been fully elucidated. The key step of conversion of 4,5-dihydrogeldanamycin to geldanamycin was expected to catalyze by a P450 monooxygenase, Gel16. The adequate bioconversions by cytochrome P450 mostly rely upon its interaction with redox partners. Several ferredoxin and ferredoxin reductases are available in the genome of certain organisms, but only a few suitable partners can operate in full efficiency. In this study, we have expressed cytochrome P450 gel16 in Escherichia coli and performed an in vitro assay using 4,5-dihydrogeldanamycin as a substrate. We demonstrated that the in silico method can be applicable for the efficient mining of convenient endogenous redox partners (9 ferredoxins and 6 ferredoxin reductases) against CYP Gel16 from Streptomyces hygroscopicus. The distances for ligand FDX4-FDR6 were found to be 9.384 Å. Similarly, the binding energy between Gel16-FDX4 and FDX4-FDR6 were -611.88 kcal/mol and -834.48 kcal/mol, respectively, suggesting the lowest distance and binding energy rather than other redox partners. These findings suggest that the best redox partners of Gel16 could be NADPH → FDR6 → FDX4 → Gel16.
목차
등록된 정보가 없습니다.
참고문헌 (41)
참고문헌 신청
Schenkman JB / 1969 / On the problem of possible other forms of cytochrome P450 in liver microsomes / Biochim. Biophys. Acta 171:23~31
Chun YJ / 2007 / Electron transport pathway for a Streptomyces cytochrome P450 : cytochrome P450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2) / J. Biol. Chem 282:17486~17500
BeBoer C / 1976 / The description and antibiotic production of Streptomyces hygroscopicus var, geldanus / J. Antibiot. (Tokyo) 29:1182~1188
Johnson RD / 1974 / Geldanamycin biosynthesis and carbon magnetic resonance / J. Am. Chem. Soc 96:3316~3317
Prodromou C / 1997 / Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone / Cell 90:65~75
Chun YJ / 2007 / Electron transport pathway for a Streptomyces cytochrome P450 : cytochrome P450 105D5-catalyzed fatty acid hydroxylation in Streptomyces coelicolor A3(2) / J. Biol. Chem 282:17486~17500
BeBoer C / 1976 / The description and antibiotic production of Streptomyces hygroscopicus var, geldanus / J. Antibiot. (Tokyo) 29:1182~1188
Johnson RD / 1974 / Geldanamycin biosynthesis and carbon magnetic resonance / J. Am. Chem. Soc 96:3316~3317
Prodromou C / 1997 / Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone / Cell 90:65~75
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Efficient extraction of intact proteins from polyacrylamide gel by using ammonium sulfate
한국분석과학회 학술대회
2021 .05
-
Spin Seebeck effect in a bulk-Y₃Fe₅O12 fabricated by a sol-gel synthesis
한국자기학회 학술연구발표회 논문개요집
2018 .03
-
Formation of Au Particles in Cu2−xICu₂ IIO3−δ (x ≈ 0.20; δ ≈ 0.10) Oxide Matrix by Sol-Gel Growth
대한화학회지
2017 .02
-
Magnetic and Dielectric Properties of CoFe₂O₄ and CoxZn1-xFe₂O₄ Nanoparticles Synthesized Using Sol-Gel Method
Journal of Magnetics
2017 .09
-
Electrolyte-Gated Artificial Synapse based on Two-dimensional material/Ion Gel Dielectric Hybrid Structure
한국진공학회 학술발표회초록집
2020 .02
-
2D Material Based Lateral-type Synaptic Device using Ion Gel Gating
한국진공학회 학술발표회초록집
2019 .08
-
비수계 레독스 플로우 배터리의 bi-radical redox pair 활용에 대한 연구
한국진공학회 학술발표회초록집
2018 .02
-
Analysis of gel containing butadiene rubber using ThFFF MALS DLS RI and gel permeation chromatography (GPC)
한국분석과학회 학술대회
2015 .05
-
Sol-gel synthesis and characterization of BaLa2WO7 phosphors
한국진공학회 학술발표회초록집
2017 .02
-
An ion-gel electrolyte based solid-state electrochromic devices
한국진공학회 학술발표회초록집
2015 .02
-
Factors Affecting Redox Potential and Differential Sensitivity of SoxR to Redox-Active Compounds
한국미생물학회 학술대회논문집
2015 .04
-
Sol-gel법을 이용한 CNT-ZnO 나노복합 구조 자외선 센서에 대한 연구
한국진공학회 학술발표회초록집
2020 .08
-
Sol-gel법을 이용한 AZO-CNT 나노복합구조 박막의 광학 및 전기적 특성에 대한 연구
한국진공학회 학술발표회초록집
2020 .08
-
Sol-gel법을 이용한 Al:ZnO계 자외선 센서의 광 반응도 향상에 관한 연구
한국진공학회 학술발표회초록집
2019 .08
-
The Fabrication of Spin-Thermoelectric Device using the Sol-Gel Method
한국자기학회 학술연구발표회 논문개요집
2015 .05
-
Sol-gel 반응 기구를 이용한 무기물 오버코팅 재료 개발
한국진공학회 학술발표회초록집
2020 .08
-
Thermoelectric voltage of bulk- Y₃Fe5O12 (YIG) prepared by the sol-gel method
한국자기학회 학술연구발표회 논문개요집
2017 .11
-
Characterization of Ni-Zn ferrite prepared by sol-gel method
한국자기학회 학술연구발표회 논문개요집
2018 .11
-
Instrumental and Sensory Correlation of Starch Gel Texture for The Elderly
동아시아식생활학회 학술발표대회논문집
2016 .10
-
Biosynthesis of Novel Glucosides Geldanamycin Analogs by Enzymatic Synthesis
Journal of Microbiology and Biotechnology
2016 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0