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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제3호
- 발행연도
- 2018.1
- 수록면
- 439 - 447 (9page)
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초록· 키워드
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The aromatic compound p-hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals, and CYP53A15 from fungus Cochliobolus lunatus is capable of executing the hydroxylation from benzoate to PHBA. Here, we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells coexpressing CYP53A15 and human NADPH-P450 oxidoreductase (CPR) genes as a redox partner. For suitable coexpression of CYP53A15 and CPR, we originally constructed five plasmids in which we replaced the N-terminal transmembrane region of CYP53A15 with a portion of the N-terminus of various mammalian P450s. PHBA productivity was the greatest when CYP53A15 expression was induced at 20°C in 2×YT medium in host E. coli strain ΔgcvR transformed with an N-terminal transmembrane region of rabbit CYP2C3. By optimizing each reaction condition (reaction temperature, substrate concentration, reaction time, and E. coli cell concentration), we achieved 90% wholecell conversion of benzoate. Our data demonstrate that the described novel E. coli bioconversion system is a more efficient tool for PHBA production from benzoate than the previously described yeast system.
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참고문헌 (29)
참고문헌 신청
Padias AB / 2011 / Mechanism studies of LCP synthesis / Polymers 3:833~845
Nelson DR / 1999 / Cytochrome P450 and the individuality of species / Arch. Biochem. Biophys. 369:1~10
Omura T / 1970 / New method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes / J. Biochem. 67:249~257
Bernhardt R / 2006 / Cytochromes P450 as versatile biocatalysts / J. Biotechnol. 124:128~145
O'Reilly E / 2011 / Cytochromes P450 as useful biocatalysts: addressing the limitations / Chem. Commun. (Camb.) 47:2490~2501
Nelson DR / 1999 / Cytochrome P450 and the individuality of species / Arch. Biochem. Biophys. 369:1~10
Omura T / 1970 / New method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes / J. Biochem. 67:249~257
Bernhardt R / 2006 / Cytochromes P450 as versatile biocatalysts / J. Biotechnol. 124:128~145
O'Reilly E / 2011 / Cytochromes P450 as useful biocatalysts: addressing the limitations / Chem. Commun. (Camb.) 47:2490~2501
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