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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 876 - 885 (10page)
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N-acetyl-D-glucosamine kinase (NAGK) plays an enzyme activity-independent, non-canonical role in the dendritogenesis of hippocampal neurons in culture. In this study, we investigated its role in axonal development. We found NAGK was distributed throughout neurons until developmental stage 3 (axonal outgrowth), and that its axonal expression remarkably decreased during stage 4 (dendritic outgrowth) and became negligible in stage 5 (mature).
Immunocytochemistry (ICC) showed colocalization of NAGK with tubulin in hippocampal neurons and with Golgi in somata, dendrites, and nascent axons. A proximity ligation assay (PLA) for NAGK and Golgi marker protein followed by ICC for tubulin or dynein light chain roadblock type 1 (DYNLRB1) in stage 3 neurons showed NAGK-Golgi complex colocalized with DYNLRB1 at the tips of microtubule (MT) fibers in axonal growth cones and in somatodendritic areas. PLAs for NAGK-dynein combined with tubulin or Golgi ICC showed similar signal patterns, indicating a three way interaction between NAGK, dynein, and Golgi in growing axons. In addition, overexpression of the NAGK gene and of kinase mutant NAGK genes increased axonal lengths, and knockdown of NAGK by small hairpin (sh) RNA reduced axonal lengths; suggesting a structural role for NAGK in axonal growth. Finally, transfection of ‘DYNLRB1 (74-96)’, a small peptide derived from DYNLRB1’s C-terminal, which binds with NAGK, resulted in neurons with shorter axons in culture. The authors suggest a NAGK-dynein-Golgi tripartite interaction in growing axons is instrumental during early axonal development.
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참고문헌 (47)
참고문헌 신청
Ahmad, F. J. / 2006 / Effects of dynactin disruption and dynein depletion on axonal microtubules / Traffic 7:524~537
Baas, P. W. / 1989 / Changes in microtubule polarity orientation during the development of hippocampal neurons in culture / J. Cell Biol 109:3085~3094
Berger, M. / 2002 / Structure and function of N-acetylglucosamine kinase. Identification of two active site cysteines / Eur. J. Biochem 269:4212~4218
Bou-Abdallah, F. / 2006 / Quenching of superoxide radicals by green fluorescent protein / Biochim. Biophys. Acta 1760:1690~1695
Brewer, G. J. / 1993 / Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination / J. Neurosci. Res 35:567~576
Baas, P. W. / 1989 / Changes in microtubule polarity orientation during the development of hippocampal neurons in culture / J. Cell Biol 109:3085~3094
Berger, M. / 2002 / Structure and function of N-acetylglucosamine kinase. Identification of two active site cysteines / Eur. J. Biochem 269:4212~4218
Bou-Abdallah, F. / 2006 / Quenching of superoxide radicals by green fluorescent protein / Biochim. Biophys. Acta 1760:1690~1695
Brewer, G. J. / 1993 / Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination / J. Neurosci. Res 35:567~576
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