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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 207 - 215 (9page)
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초록· 키워드
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Mosquito-borne tropical dengue disease is a major global epidemic affecting hundreds of millions of people. As a precaution, it would be ideal to develop a highly efficient tetravalent vaccine that protects against all serotypes of dengue viruses. In this study, envelope protein domain III (EDIII) of dengue virus was employed as a target antigen. A single-chain tetravalent type of EDIII (tEDIII) was generated by utilizing a linker peptide and transformed into rice (Oryza sativa). Integration and mRNA transcripts of the tEDIII gene were confirmed by genomic DNA polymerase chain reaction (PCR) and Northern blot analyses, respectively. The expression of wild-type tEDIII (wtEDIII) protein was confirmed by Western blot analysis and was determined to improve the synthesis of the tEDIII (stEDIII) construct based on codon optimization and ER targeting. The yield of stEDIII protein was sevenfold higher than secreted wtEDIII protein, reaching a maximum of 357 μg per gram dry weight. These results suggest that a simple tetravalent EDIII dengue antigen can be produced in rice, raising the possibility that edible plant cells can be vaccinated by mucosal application for protection against dengue infection.
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참고문헌 (72)
참고문헌 신청
Adang MJ / 1993 / The reconstruction and expression of a Bacillus thuringiensis cryIIIA gene in protoplasts and potato plants / Plant Mol Biol 21(6):1131~1145
Apt D / 2006 / Tetravalent neutralizing antibody response against four dengue serotypes by a single chimeric dengue envelope antigen / Vaccine 24(3):335~344
Arakawa T / 1997 / Expression of cholera toxin B subunit oligomers in transgenic potato plants / Transgenic Res 6(6):403~413
Block OK / 2010 / A tetravalent recombinant dengue domain III protein vaccine stimulates neutralizing and enhancing antibodies in mice / Vaccine 28(51):8085~8094
Brandler S / 2010 / Pediatric measles vaccine expressing a dengue tetravalent antigen elicits neutralizing antibodies against all four dengue viruses / Vaccine 28(41):6730~6739
Apt D / 2006 / Tetravalent neutralizing antibody response against four dengue serotypes by a single chimeric dengue envelope antigen / Vaccine 24(3):335~344
Arakawa T / 1997 / Expression of cholera toxin B subunit oligomers in transgenic potato plants / Transgenic Res 6(6):403~413
Block OK / 2010 / A tetravalent recombinant dengue domain III protein vaccine stimulates neutralizing and enhancing antibodies in mice / Vaccine 28(51):8085~8094
Brandler S / 2010 / Pediatric measles vaccine expressing a dengue tetravalent antigen elicits neutralizing antibodies against all four dengue viruses / Vaccine 28(41):6730~6739
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