Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus

In-Ohk Ouh; Ju-Yeon Lee; Seo Young Moon; Jae Young Song; Sang Ho Jang; Kwang Myun Cheong; Choi-Kyu Park; Yoon-Hee Lee

Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus

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Type: Academic journal

Author: In-Ohk Ouh (농림축산검역본부) Ju-Yeon Lee (Viral Disease Division Animal and Plant Quarantine Agency) Seo Young Moon (농림축산검역본부) Jae Young Song (농림축산검역본부) Sang Ho Jang (Mediandignostic Inc) Kwang Myun Cheong (Mediandignostic Inc) Choi-Kyu Park (경북대학교 수의과대학) Yoon-Hee Lee (농림축산검역본부)

Journal: The Korean Society of Preventive Veterinary Medicine 예방수의학회지 예방수의학회지 제45권 제4호 KCI Accredited Journals

Published: 2021.12

Pages: 158 - 165 (8page)

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Improved solid-phase competition enzyme-linked immunosorbent assay for the specific detection of antibodies against porcine parvovirus based on VP2 protein expressed in baculovirus

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A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.

#porcine parvovirus (PPV) #enzyme-linked immunosorbent assay (ELISA) #VP2

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References (27)

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